• Home
  • Search Results
  • Efficient reprogramming of mouse fibroblasts to neuronal cells including dopaminergic neurons.

Efficient reprogramming of mouse fibroblasts to neuronal cells including dopaminergic neurons.

TheScientificWorldJournal (2014-07-06)
Seung-ick Oh, Hang-soo Park, Insik Hwang, Han-kyul Park, Kyung-Ah Choi, Hyesun Jeong, Suhng Wook Kim, Sunghoi Hong
ABSTRACT

Somatic cells were directly converted to functional neurons through the use of a combination of transcription factors, including Ascl1, Brn2, and Myt1l. However, a major limitation is the lack of a reliable source of cell-replacement therapy for neurological diseases. Here, we show that a combination of the transcription factors Ascl1 and Nurr1 (AN) and neurotrophic factors including SHH and FGF8b directly reprogrammed embryonic mouse fibroblasts to induced neuronal (iN) cells: pan-neuronal cells and dopaminergic (DA) neurons under our systematic cell culture conditions. Reprogrammed cells showed the morphological properties of neuronal cells. Additionally, cells were analyzed using various markers, including Tuj1 and Map2 for neuronal cells and Lmx1a, Th, Aadc and Vmat2 for DA neurons in our immunostaining and reverse transcription (RT)-PCR experiments. We found that a combination of transcription factors and neurotrophic factors could directly reprogram fibroblasts to neuronal cells including DA neurons. Various types of reprogrammed cells are promising cell sources for cell-based therapy of neurological disorders like Parkinson's disease and spinal cord injury.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Tyrosine Hydroxylase Antibody, Chemicon®, from rabbit
Sigma-Aldrich
TAPS, ≥99.5% (titration)
Sigma-Aldrich
Anti-Nestin Antibody, clone 10C2, clone 10C2, Chemicon®, from mouse
Sigma-Aldrich
TAPS, BioPerformance Certified, suitable for cell culture, ≥99.5% (titration)
Sigma-Aldrich
Monoclonal Anti-Vimentin antibody produced in mouse, clone VIM-13.2, ascites fluid
Sigma-Aldrich
TAPS, BioXtra, ≥99.5% (titration)
Sigma-Aldrich
Monoclonal Anti-MAP2 (2a+2b) antibody produced in mouse, ~2 mg/mL, clone AP-20, purified immunoglobulin, buffered aqueous solution

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.