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Synthesis and in vitro localization study of curcumin-loaded SPIONs in a micro capillary for simulating a targeted drug delivery system.

International journal of pharmaceutics (2014-04-22)
Mohammed Anwar, Mohammed Asfer, Ayodhya P Prajapati, Sharmistha Mohapatra, Sohail Akhter, Asgar Ali, Farhan J Ahmad
ABSTRACT

Nano-sized curcumin-loaded super-paramagnetic iron oxide nanoparticles (CUR-OA-SPIONs) were synthesized chemically by co-precipitation method using oleic acid as a stabilizer and Myrj 52 as a surfactant. The synthesized nanoparticles were characterized for their shape, size, surface morphology, electrokinetic potential, magnetic properties, crystalinity, chemical interactions and thermal transitions. The synthesized CUR-OA-SPIONs were spherical, mono-dispersed, physically stable and super-paramagnetic in nature. In vitro localization study and aggregation dynamics of CUR-OA-SPIONs were studied with a flow of blood inside a square glass capillary (500×500 μm(2) cross section) in the presence of an externally applied magnetic field (Ms=1200 mT). This research which is first of its kind showed the fluorescent imaging of CUR-OA-SPIONs with respect to time to understand the aggregation dynamics of magnetic nanoparticles in a micro capillary simulating the case of targeted drug delivery system. The size of the aggregation increases with respect to time (t=0(+)s to t=500 s), while no significant change in the size of the aggregate was observed after time t=500 s.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ammonium hydroxide solution, ACS reagent, 28.0-30.0% NH3 basis
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Hexane, suitable for HPLC, ≥97.0% (GC)
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Hexane, anhydrous, 95%
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Oleic acid, ≥99% (GC)
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Curcumin, from Curcuma longa (Turmeric), powder
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Hexane, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99% (GC)
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Oleic acid, technical grade, 90%
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Ammonium hydroxide solution, 28% NH3 in H2O, ≥99.99% trace metals basis
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Hexane, suitable for HPLC, ≥95%
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Hexane, HPLC Plus, for HPLC, GC, and residue analysis, ≥95%
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Oleic acid, suitable for cell culture, BioReagent
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Hexane, ReagentPlus®, ≥99%
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Hexane, Laboratory Reagent, ≥95%
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Iron(III) chloride, anhydrous, powder, ≥99.99% trace metals basis
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Curcumin, ≥94% (curcuminoid content), ≥80% (Curcumin)
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Oleic acid, analytical standard
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Ammonium hydroxide solution, puriss. p.a., reag. ISO, reag. Ph. Eur., ~25% NH3 basis
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Ammonium hydroxide solution, BioUltra, ~1 M NH3 in H2O (T)
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Oleic acid, natural, FCC
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Ammonium hydroxide solution, puriss., 30-33% NH3 in H2O
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Iron(III) chloride solution, purum, 45% FeCl3 basis
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Hexane, analytical standard
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Iron(III) chloride, sublimed grade, ≥99.9% trace metals basis
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Curcumin, matrix substance for MALDI-MS, ≥99.5% (HPLC)
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Ammonium hydroxide solution, puriss., meets analytical specification of Ph. Eur., 25-30% NH3 basis
USP
Curcumin, United States Pharmacopeia (USP) Reference Standard
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Ammonium hydroxide solution, puriss. p.a. plus, ≥25% NH3 in H2O
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TDA Reagent, suitable for microbiology
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Oleic acid, meets analytical specification of Ph, Eur., 65.0-88.0% (GC)