MilliporeSigma
  • Selective Capture and Quick Detection of Targeting Cells with SERS-Coding Microsphere Suspension Chip.

Selective Capture and Quick Detection of Targeting Cells with SERS-Coding Microsphere Suspension Chip.

Small (Weinheim an der Bergstrasse, Germany) (2015-01-20)
Dian Li, Yuting Zhang, Ruimin Li, Jia Guo, Changchun Wang, Chuanbing Tang
ABSTRACT

Circulating tumor cells (CTCs) captured from blood fluid represent recurrent cancers and metastatic lesions to monitor the situation of cancers. We develop surface-enhanced Raman scattering (SERS)-coding microsphere suspension chip as a new strategy for fast and efficient capture, recovery, and detection of targeting cancer cells. Using HeLa cells as model CTCs, we first utilize folate as a recognition molecule to be immobilized in magnetic composite microspheres for capturing HeLa cells and attaining high capturing efficacy (up to 95%). After capturing cells, the composite microsphere, which utilizes a disulfide bond as crosslinker in the polymer shell and as a spacer for linking folate, can recycle 90% cells within 20 min eluted by glutathion solution. Taking advantage of the SERS with fingerprint features, we characterize captured/recovered cells with the unique signal of report-molecule 4-aminothiophenol through introducing the SERS-coding microsphere suspension chip to CTCs. Finally, the exploratory experiment of sieving cells shows that the magnetic composite microspheres can selectively capture the HeLa cells from samples of mixed cells, indicating that these magnetic composite microspheres have potential in real blood samples for capturing CTCs.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Styrene, ReagentPlus®, contains 4-tert-butylcatechol as stabilizer, ≥99%
Sigma-Aldrich
2,2′-Azobis(2-methylpropionitrile), recrystallized from methanol, 99%
Sigma-Aldrich
2,2′-Azobis(2-methylpropionitrile), 98%
Sigma-Aldrich
Triethoxysilane, 95%
Sigma-Aldrich
Acrylic acid, anhydrous, contains 200 ppm MEHQ as inhibitor, 99%
Sigma-Aldrich
Cystamine dihydrochloride, BioXtra
Sigma-Aldrich
N-Hydroxysuccinimide, 98%
Sigma-Aldrich
Cystamine dihydrochloride, 96%