Toluidine blue is a basic thiazine metachromatic dye that binds to nuclear material of tissues with a high DNA and RNA content and is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals. This effective staining agent is used for identifying the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
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