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For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
40718
Diethyl pyrocarbonate
96% (NT)
Synonym(s):
DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
5 mL | Available to ship TODAYfromMILWAUKEE | $96.00 |
25 mL | Available to ship TODAYfromMILWAUKEE | $191.00 |
100 mL | Available to ship TODAYfromMILWAUKEE | $628.00 |
250 mL | Check Cart for Availability | $1,150.00 |
About This Item
Quality Segment
assay
96% (NT)
form
liquid
color
APHA: ≤20
refractive index
n20/D 1.398
bp
93-94 °C/18 mmHg (lit.)
solubility
95% ethanol: 4.5 g/10 mL, clear, colorless
density
1.121 g/mL at 20 °C, 1.101 g/mL at 25 °C (lit.)
functional group
carbonate
shipped in
wet ice
storage temp.
2-8°C
SMILES string
CCOC(=O)OC(=O)OCC
InChI
1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
InChI key
FFYPMLJYZAEMQB-UHFFFAOYSA-N
General description
Application
- For the modification of histidyl residues in proteins.
- As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
- For the modification of linear and supercoiled plasmid DNAs.
- As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
- For cabethoxylation of histidine residues of actin.
Biochem/physiol Actions
1 of 1
This Item | |||
|---|---|---|---|
| description 96% (NT) | description 96% (NT) | description 96% (NT) | description Pharmaceutical Secondary Standard; Certified Reference Material |
| assay 96% (NT) | assay 96% (NT) | assay 96% (NT) | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| solubility 95% ethanol: 4.5 g/10 mL, clear, colorless | solubility - | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. -10 to -25°C |
| refractive index n20/D 1.398 | refractive index n20/D 1.398 (lit.) | refractive index n20/D 1.398 (lit.) | refractive index - |
| bp 93-94 °C/18 mmHg (lit.) | bp 93-94 °C/18 mmHg (lit.) | bp 93-94 °C/18 mmHg (lit.) | bp - |
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Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
156.2 °F - closed cup
Flash Point (°C)
69 °C - closed cup
PPE (personal protective equipment)
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the solubility and solution stability of Diethyl pyrocarbonate, DEPC?
1 answer-
DEPC can be solubilized in 95% ethanol, e.g. 1.6 mL in 4 mL ethanol gives a clear, colorless solution. DEPC is soluble in alcohols, esters, ketones and other hydrocarbons, but has limited solubility in water (~0.1%). 6 DEPC hydrolyzes in aqueous solution, to form ethanol and carbon dioxide (CO2). Typically a 0.1% DEPC solution (1 mL of DEPC diluted in water to 1 L) is used to inactivate RNase. The DEPC will not immediately dissolve, as evidenced by the appearance of globules. The mixture should be stirred until the globules disappear. DEPC is sensitive to pH. In phosphate buffer at 25 °C, its half-life is 4 minutes at pH 6, and 9 minutes at pH 7. Hydrolysis is accelerated by Tris buffer,7 where at 25 °C, the half-life of DEPC was reported to be 1.25 minutes at pH 7.5, and 0.37 minutes at pH 8.2. DEPC will decompose in solution when autoclaved. For 0.1% DEPC solutions, autoclaving for 15 minutes per liter should be sufficient.
DEPC is very sensitive to moisture. As such, DEPC is packaged under argon to help reduce exposure to moisture. If DEPC is exposed to even traces of moisture, some hydrolysis occurs. The resulting CO2 is more soluble in DEPC solutions at 2–8 °C than at room temperature. As the product is brought to room temperature before opening, the DEPC can become supersaturated with respect to any dissolved gas. After opening the bottle for the first time and each time thereafter, layer nitrogen or argon gas over the DEPC and store the closed bottle at 2–8 °C for optimal stability. It may be helpful to store the bottle inside a sealed plastic bag with desiccant, but with the bottle cap slightly loose. If the bag inflates at all, this indicates some degree of decomposition with possible pressure build-up. Once opened, the bottle should not be kept for more than 9 months. DEPC decomposes at 155 °C. DEPC is also sensitive to ammonia, which causes decomposition to urethane.Helpful?
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