SCT033

BioTracker 520 Green Hypoxia Dye

Live cell imaging dye for hypoxia that increases in fluorescence intensity with decreasing oxygen levels.

• Synonym(s): Live cell hypoxia probe, Live cell imaging probe
• UNSPSC Code: 12352207
• NACRES: NA.47
• eCl@ss: 32161000

Properties

• technique(s): cell based assay: suitable, live cell imaging : suitable
• detection method: fluorometric
• shipped in: ambient
• storage temp.: -10 to -25°C

Description

General description

Hypoxia is defined as the reduction or lack of oxygen in organs, tissues, or cells. Hypoxia in living tissue closely relates to a wide array of pathologies, including cancer and ischemia. Existing hypoxia detection probes, such as pimonidazole, require cell fixation followed by immunostaining.

The BioTracker 520 Green Hypoxia Dye is a fluorescent imaging probe for the detection of hypoxia in living cells. This probe is as sensitive as pimonidazole and can be used for live cell fluorescence imaging and flow cytometry applications.

Spectral Properties
Absorbance: 498nm
Emission: 520nm

Application

Live cell fluorescent imaging

Research Category
Cell Imaging

Research Sub Category
Live Cell Dye

Physical form

Lyophilized

Preparation Note

Store BioTracker 520 Green Hypoxia Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable