CLL1219

Safe Harbor Landing Pad Cell Line A375 Cancer Cells

human female skin (Source Disease: Malignant melanoma)

• UNSPSC Code: 41106514
• NACRES: NA.81
• Biological source: human female skin (Source Disease: Malignant melanoma)
• Growth mode: Adherent

Properties

• Product Name: Safe Harbor Landing Pad Cell Line A375 Cancer Cells,
• biological source: human female skin (Source Disease: Malignant melanoma)
• Quality Segment: 200
• growth mode: Adherent
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

The STR profile of this cell line matches that of its parental cell line ATCC^® Catalog No. CRL-1619. A375 are a human malignant melanoma cell line isolated from a 54 year old female. The cells possess a hypotriploid karyotype.

Application

This product is a human A375 melanoma cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr^® Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user′s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.

Biochem/physiol Actions

These cells are a human malignant melanoma cell line isolated from a 54-year old female. The cells possess a hypotriploid karyotype.

Features and Benefits

These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These A375 cells are adherent with a doubling time of approximately 22 hours.

Preparation Note

DMEM (Catalog Number D6429) with 10% FBS (Catalog Number F2442)

Analysis Note

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Legal Information

ATCC is a registered trademark of American Type Culture Collection

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable