CLL1221

Safe Harbor Landing Pad Cell Line Jurkat T-Lymphocytes

human male peripheral blood (Source Disease: Acute T cell leukemia)

• Synonym(s): T-Lymphocyte Cell Line
• UNSPSC Code: 41106514
• NACRES: NA.81
• Biological source: human male peripheral blood (Source Disease: Acute T cell leukemia)
• Growth mode: Suspension

Properties

• Product Name: Safe Harbor Landing Pad Cell Line Jurkat T-Lymphocytes,
• biological source: human male peripheral blood (Source Disease: Acute T cell leukemia)
• Quality Segment: 200
• form: frozen liquid (Vial of Frozen Cells)
• growth mode: Suspension
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

The STR profile of this cell line matches that of its parental cell line ATCC^® Catalog No. TIB-152. Jurkat T lymphocytes are a human, acute T cell lymphoma cell line isolated in the late 1970s from the peripheral blood of a young male patient suffering from T cell leukemia. The cells possess a pseudodiploid karyotype and have been characterized as expressing CD3 and, upon stimulation, interleukin-2.

Application

This product is a human Jurkat T-lymphocyte cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr^® Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user′s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.

Biochem/physiol Actions

These cells are a human, acute T cell lymphoma cell line isolated from the peripheral blood of a young male suffering from T cell leukemia. The cells possess a pseudodiploid karyotype, express CD3 and, upon stimulation, IL-2.

Features and Benefits

These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These Jurkat cells are grown in suspension with a doubling time of approximately 22 hours.

Preparation Note

RPMI modified to contain 2mM L-glutamine, 10mM HEPES, 1mM sodium pyruvate, and 1500mg/L sodium bicarbonate. Add 10% FBS(Catalog Number F2442).

Analysis Note

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Legal Information

ATCC is a registered trademark of American Type Culture Collection

CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Codes: H290
• Precautionary Statements: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• Storage Class: 10 - Combustible liquids