CLLS1014
A549 Cells HIF1A -/-/-
human male lung (Source Disease: Lung carcinoma)
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| Size/SKU | Availability | Price |
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1 set | Check Cart for Availability | $5,030.00 |
About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Biological source:
human male lung (Source Disease: Lung Carcinoma)
Product Name
A549 Cells HIF1A -/-/-,
biological source
human male lung (Source Disease: Lung Carcinoma)
Quality Segment
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... HIF1a(3091)
General description
A549 Cells HIF1A -/-/- are lung carcinoma, epithelial cells, from a human caucasian male (aged 58 years), with a ZFN knock out modification.
This product corresponds to ATCC Cat. No. CCL-185
Application
Media Renewal changes two to three times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3 to 1:8
The base medium for this cell line is F12 Ham Medium, Cat. No. N4888. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10% and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
The cell freezing medium is DMSO 1X (Cat. No. C6164)
Features and Benefits
These A549 cells are adherent, with a doubling time of approx. 22 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 14q23.2
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
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This Item | |||
|---|---|---|---|
| description - | description - | description - | description - |
| biological source human male lung (Source Disease: Lung Carcinoma) | biological source human female colon (Source disease: Colorectal adenocarcinoma) | biological source human male lung (Source Disease: Lung carcinoma) | biological source human male lung (Source Disease: Lung carcinoma) |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| Gene Information human ... HIF1a(3091) | Gene Information human ... HIF1A(3091) | Gene Information human ... STAT1(6772) | Gene Information human ... SMAD4(4089) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| OMIM accession no. | OMIM accession no. - | OMIM accession no. | OMIM accession no. |
Kit Components Only
Product No.
Description
- A549 Cells HIF1A -/-/- 1 vial
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