CLLS1049
MCF10A CELLS TP53 (-/-)
human female mammary glands (Source Disease: Fibrocystic Disease)
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| Size/SKU | Availability | Price |
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1 set | Check Cart for Availability | $5,030.00 |
About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
eCl@ss:
32190102
Biological source:
human female mammary glands (Source Disease: Fibrocystic Disease)
Product Name
MCF10A CELLS TP53 (-/-),
biological source
human female mammary glands (Source Disease: Fibrocystic Disease)
Quality Segment
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... TP53(7157)
General description
MCF10A CELLS TP53 (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.
Application
Cell freezing medium-DMSO 1X, Cat. No. C6164
Media renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.
Features and Benefits
These MCF10A cells are adherent with a doubling time of approx. 20 hours
Zinc finger nuclease (ZFN) knockout on chromosome 17p13.1
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
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This Item | |||
|---|---|---|---|
| description - | description - | description - | description - |
| biological source human female mammary glands (Source Disease: Fibrocystic Disease) | biological source human female mammary glands (Source Disease: Fibrocystic Disease) | biological source human female mammary glands (Source Disease: Fibrocystic Disease) | biological source human female mammary glands (Source Disease: Fibrocystic Disease) |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| Gene Information human ... TP53(7157) | Gene Information human ... APC(324) | Gene Information human ... ESR2(2100) | Gene Information human ... AKT2(208) |
| OMIM accession no. | OMIM accession no. | OMIM accession no. | OMIM accession no. |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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