MREH01
MISSION® Target ID Library
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| Size/SKU | Availability | Price |
|---|---|---|
1 set | Check Cart for Availability | $4,290.00 |
About This Item
NACRES:
NA.51
UNSPSC Code:
41105904
Concentration:
1 ng/μL (library), 25 μM (primer 1), 25 μM (primer 2)
Quality Segment
packaging
pkg of 100 μL (library), pkg of 200 μL (primer 1), pkg of 200 μL (primer 2)
concentration
1 ng/μL (library), 25 μM (primer 1), 25 μM (primer 2)
shipped in
wet ice
storage temp.
−20°C
General description
The Target ID Library enables bench-top transcriptome-wide human miRNA and ncRNA gene target identification. It has an innovative dual selection system, which makes rapid whole transcriptome miRNA and ncRNA gene target screens accessible to any researcher with minimal reagent, time, or capital equipment expense.
The Target ID Library is a transcriptome wide cDNA pool produced using a proprietary process. Each library is tested for gene representation to ensure robust library coverage. The library is provided in ready-to-use DNA format, with enough material for multiple genome-wide screens.
The Target ID Library is a pool of plasmids, each with a human cDNA inserted into the 3′-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencing
Download deep sequencing data of target ID library.
The Target ID Library is a transcriptome wide cDNA pool produced using a proprietary process. Each library is tested for gene representation to ensure robust library coverage. The library is provided in ready-to-use DNA format, with enough material for multiple genome-wide screens.
The Target ID Library is a pool of plasmids, each with a human cDNA inserted into the 3′-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencing
Download deep sequencing data of target ID library.
Other Notes
Library: 100μl (approx 1μg/μl), frozen liquid, store at -10°C to -25°C.
Primer 1: 200μl (25μM), frozen liquid, store at -10°C to -25°C
Primer 2: 200μl (25μM), frozen liquid, store at -10°C to -25°C
Primer 1: 200μl (25μM), frozen liquid, store at -10°C to -25°C
Primer 2: 200μl (25μM), frozen liquid, store at -10°C to -25°C
Legal Information
MISSION is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| description - | description For Rapid, Convenient Genome-wide shRNA Screens | description For Rapid, Convenient Genome-wide shRNA Screens | description For Rapid, Convenient Genome-wide shRNA Screens |
| concentration 1 ng/μL (library), 25 μM (primer 1), 25 μM (primer 2) | concentration ≥5x108 VP/ml (via p24 assay) | concentration ≥5x108 VP/ml (via p24 assay) | concentration ≥5x108 VP/ml (via p24 assay) |
| storage temp. −20°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| packaging pkg of 200 μL (primer 1), pkg of 200 μL (primer 2) | packaging - | packaging - | packaging - |
Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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