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Peroxidase from horseradish

Name: Peroxidase from horseradish
Brand: SIGMA
Price: 106 USD
Availability: InStock

Type X, ammonium sulfate suspension

Synonym(s):

Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

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Size/SKU	Availability	Price
2000 units	Estimated to ship TODAYfromSAINT LOUIS	$106.00
5000 units	Estimated to ship TODAYfromSAINT LOUIS	$183.00
25000 units	Estimated to ship onOctober 20, 2026fromSAINT LOUIS	$667.00

About This Item

CAS Number:

9003-99-0

eCl@ss:

32160410

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-668-6

MDL number:

MFCD00071339

EC Number:

1.11.1.7 (BRENDA, IUBMB)

Specific activity:

≥225 units/mg protein (biuret, using pyrogallol)

Biological source:

horseradish

Concentration:

≥1.0 mg/mL

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Properties

biological source

horseradish

Quality Segment

200

type

Type X

form

ammonium sulfate suspension

specific activity

≥225 units/mg protein (biuret, using pyrogallol)

mol wt

~44 kDa

concentration

≥1.0 mg/mL

technique(s)

protein detection: suitable

absorbance ratio

RZ 2.5-3.5

storage temp.

2-8°C

SMILES string

[O+H2]O[O-]

InChI

1S/H2O3/c1-3-2/h1-2H

InChI key

JSPLKZUTYZBBKA-UHFFFAOYSA-N

Description

General description

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca²⁺ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Application

Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P6140 has been used to detect low density lipoprotein (LDL).

The enzyme from Sigma has been used while assessing the skin sensitization potential of pro-haptens. It has also been used to show that peroxidase (PO) activity and its heat stability correlate with the availability of free Ca²⁺ ions.

Biochem/physiol Actions

When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. Horseradish peroxidase has been shown to slightly reduce the level of inhibition in a cydAB mutant.

HRP readily combines with hydrogen peroxide (H₂O₂) and the resultant [HRP-H₂O₂] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies by several different methods that include the use of glutaraldehyde, periodate oxidation, disulfide bonds, and also via amino- and thiol-directed cross-linkers. It is smaller and more stable than the enzyme labels, β-galactosidase and alkaline phosphatase. Hence, it is the most desired label. Also, its glycosylation leads to lower non-specific binding. It is also used for the determination of glucose and peroxides in solution. Sodium azide, cyanide, L-cystine, dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd²⁺, Co²⁺, Cu²⁺, Fe³⁺, Mn²⁺, Ni²⁺, Pb²⁺ ions are found to inhibit its enzyme activity.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄ solution containing potassium phosphate buffer, pH 6.0

Preparation Note

Water may be used to dilute suspension if needed.

Analysis Note

Preliminary studies indicate the presence of two basic and no acidic isoenzymes

The RZ (Reinheitszahl) is the absorbance ratio A₄₀₃/A₂₇₅ determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.

Other Notes

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer.

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Show Differences

1 of 1

This Item	77332	P8250	P8415
Sigma-Aldrich P6140 Peroxidase from horseradish	Sigma-Aldrich 77332 Peroxidase from horseradish	Sigma-Aldrich P8250 Peroxidase from horseradish	Sigma-Aldrich P8415 Peroxidase from horseradish
description Type X, ammonium sulfate suspension	description lyophilized, powder, ~150 U/mg	description Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid (using pyrogallol)	description Type XII, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)
biological source horseradish	biological source horseradish	biological source horseradish	biological source -
technique(s) protein detection: suitable	technique(s) protein detection: suitable	technique(s) protein detection: suitable	technique(s) protein detection: suitable
specific activity ≥225 units/mg protein (biuret, using pyrogallol)	specific activity ~150 U/mg	specific activity 150-250 units/mg solid (using pyrogallol)	specific activity ≥250 units/mg solid (using pyrogallol)
form ammonium sulfate suspension	form powder	form essentially salt-free, lyophilized powder	form essentially salt-free, lyophilized powder
concentration ≥1.0 mg/mL	concentration -	concentration -	concentration -
mol wt ~44 kDa	mol wt -	mol wt ~44 kDa	mol wt ~44 kDa

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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