The recommended storage conditions for this antibody are as follows: Store the lyophilized product at 2–8°C. For extended storage after reconstitution, keep at –20°C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8°C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.
SAB4200768
Anti-Human IgG1-Peroxidase antibody, Mouse monoclonal
clone 8c/6-39, purified from hybridoma cell culture
Synonym(s):
Anti-Human immunoglobulin G1
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| Size/SKU | Availability | Price |
|---|---|---|
1 vial | Check Cart for Availability | $473.00 |
About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
8c/6-39, monoclonal
Application:
ELISA
Citations:
2
Quality Segment
biological source
mouse
conjugate
peroxidase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
8c/6-39, monoclonal
form
lyophilized
species reactivity
human
concentration
~2 mg/mL
technique(s)
ELISA: 1:8,000-1:16,000 using 5 μg/ml human IgG1 for coating
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Human IgGs consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5, and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties.Monoclonal Anti-Human IgG1 specifically recognizes Human IgG1. The antibody shows no cross-reactivity with human IgG2, IgG3, or IgG4. This clone has been established as a useful human IgG1 specificity standard by the WHO/IUIS.
Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the 8c/6-39 hybridoma (also known as HP6019), produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with the Fc fragment of a human IgG1 myeloma protein.
Immunogen
The Fc fragment of a human IgG1 myeloma protein
Application
The antibody is recommended to use in various immunological techniques, including ELISA.
Biochem/physiol Actions
The immunoglobin G (IgG) subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies. Human IgG1 is the main antibody produced against tetanus toxoid antibodies. Elevation of IgG1 levels has been found in the cerebral spinal fluid of patients with multiple sclerosis.
Physical form
Supplied as lyophilized powder. After reconstitution with 0.1 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
1 of 1
This Item | |||
|---|---|---|---|
| description clone 8c/6-39, purified from hybridoma cell culture | description clone HP-6050, purified from hybridoma cell culture | description clone HP-6001, purified from hybridoma cell culture | description clone HP-6025, purified from hybridoma cell culture |
| antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone 8c/6-39, monoclonal | clone HP-6050, monoclonal | clone HP-6001, monoclonal | clone HP-6025, monoclonal |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate - | conjugate peroxidase conjugate |
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| technique(s) ELISA: 1:8,000-1:16,000 using 5 μg/ml human IgG1 for coating | technique(s) direct ELISA: 1:80,000-1:160,000 using 2 μg/mL human IgG3 for coating. | technique(s) indirect ELISA: 0.5 μg/mL using 1 μg/mL Human IgG1 myeloma protein for coating. | technique(s) ELISA: 1:80,000-1:160,000 using 1 μg/mL human IgG4 for coating |
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Could a lower temperature, such as -80°C, be used for storage during the reconstitution of SAB4200768 instead of the recommended -20°C?
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