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809705

5-DBCO-PEG4-dUTP, 10mM Aqueous Solution

Synonym(s):

5-DBCO-PEG4-dUTP

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Pack SizeSKUAvailabilityPrice
50 μL
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$441.00

About This Item

Empirical Formula (Hill Notation):
C42H54N5O21P3
Molecular Weight:
1057.82
UNSPSC Code:
12352200
PubChem Substance ID:
329769180
NACRES:
NA.22
Assay:
>95% (HPLC)
Form:
solution

$441.00

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assay

>95% (HPLC)

Quality Level

200

form

solution

reaction suitability

reaction type: click chemistry

solubility

Tris buffer: 100 mM (pH 7.5)

shipped in

wet ice

storage temp.

−20°C

SMILES string

O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O

InChI

1S/C42H54N5O21P3/c48-35-26-40(66-36(35)29-65-70(57,58)68-71(59,60)67-69(54,55)56)47-28-33(41(52)45-42(47)53)9-5-16-43-38(50)15-18-61-20-22-63-24-25-64-23-21-62-19-17-44-37(49)13-14-39(51)46-27-32-8-2-1-6-30(32)11-12-31-7-3-4-10-34(31)46/h1-10,28,35-36,40,

InChI key

DINRDGNWAWZURW-AWZRJCEASA-N

Application

5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.

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This Item
764019808520808512
5-DBCO-PEG4-dUTP, 10mM Aqueous Solution

Sigma-Aldrich

809705

5-DBCO-PEG4-dUTP, 10mM Aqueous Solution

Dibenzocyclooctyne-PEG4-N-hydroxysuccinimidyl ester ≥90%

Sigma-Aldrich

764019

Dibenzocyclooctyne-PEG4-N-hydroxysuccinimidyl ester

γ-[(6-Azidohexyl)-imido]-ATP sodium salt

Sigma-Aldrich

808520

γ-[(6-Azidohexyl)-imido]-ATP sodium salt

γ-[(Propargyl)-imido]-ATP sodium salt

Sigma-Aldrich

808512

γ-[(Propargyl)-imido]-ATP sodium salt

assay

>95% (HPLC)

assay

≥90%

assay

≥95% (HPLC)

assay

≥95% (HPLC)

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

solubility

Tris buffer: 100 mM (pH 7.5)

solubility

-

solubility

10 mM Tris-HCl, pH 7.5: soluble

solubility

10 mM Tris-HCl, pH 7.5: soluble

reaction suitability

reaction type: click chemistry

reaction suitability

reaction type: click chemistry, reagent type: linker

reaction suitability

reaction type: click chemistry

reaction suitability

reaction type: click chemistry

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

form

solution

form

paste

form

powder

form

solid

Storage Class

10 - Combustible liquids

wgk

WGK 2

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Certificates of Analysis (COA)

Lot/Batch Number
0000362905
0000362905
SLCQ5204
SLCK9929
SLCH5980

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Global Trade Item Number

SKUGTIN
809705-50UL04061838041067

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