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Coupling Reagents and Nucleosides

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Oligo synthesis cycle A

Stepwise overview of the oligonucleotide synthesis cycle, highlighting the key stages: deblocking, coupling, capping, oxidation, and final processing or initiation of the next cycle.

Oligo Synthesis Cycle B

Illustration of the phosphoramidite oligonucleotide synthesis process, showing the chemical transformations during deprotection, coupling, capping of uncoupled bases, and oxidation

We offer a collection of useful building blocks, coupling reagents, and nucleosides for applications in oligo synthesis and beyond. Nucleosides and modified nucleosides are the basic structural units that make up nucleic acids (DNA and RNA), which are responsible for encoding, transmitting and expressing genetic information in all living things. Oligonucleotide synthesis outside of biological systems is the chemical synthesis of nucleic acids of a predetermined sequence that may contain nucleosides beyond those that are naturally occurring. Typical oligonucleotide synthesis using nucleoside phosphoramidites occurs in a four-step process: deblocking, coupling, capping, and oxidation. Coupling reagents play an important role in these steps, efficient coupling activators assuring the reactions are fast and nearly quantitative. 

The ability to incorporate non-natural nucleosides into nucleic acids has led to many research uses such as:

  • sequence detection 
  • hydrolysis resistance
  • sequencing
  • enzyme inhibition

Nucleoside chemistry has produced non-natural analogs that have been useful in medicine with chemotherapeutic and antiviral applications, such as reverse transcriptase inhibition. Our selection of unnatural bases, nucleoside derivatives and intermediates will provide you with a useful starting point for your oligo or nucleoside analog synthesis needs.

Products

Acetylations (26)

click chemistry (8)

Oxidations (7)

Coupling Reactions (5)

Aminolysis (2)

Buchwald-Hartwig Cross Coupling Reaction (2)

derivatization reagent (19)

Lewis acid (7)

oxidant (7)

catalyst (5)

Lewis base (2)

ligand (2)

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Adenosine 5′-triphosphate disodium salt hydrate
A26209

Adenosine 5′-triphosphate disodium salt hydrate

99%

Deblocking Reagent 3% Dichloroacetic Acid in Dichloromethane
BI0833

Deblocking Reagent 3% Dichloroacetic Acid in Dichloromethane

Novabiochem®

Oxidizing Reagent, 0.05M, I<sub>2</sub> in Pyridine
BI0424

Oxidizing Reagent, 0.05M, I2 in Pyridine

Biosynthesis Novabiochem®

5-Ethynyl-2′-deoxyuridine
900584

5-Ethynyl-2′-deoxyuridine

95%

Activator Solution 0.25M 5-(Ethylthio)-1H-Tetrazole in Acetonitrile
BI0152

Activator Solution 0.25M 5-(Ethylthio)-1H-Tetrazole in Acetonitrile

Biosynthesis Novabiochem®

Deblocking Reagent 3% Dichloroacetic Acid in Toluene
BI0832

Deblocking Reagent 3% Dichloroacetic Acid in Toluene

Novabiochem®

Activator Reagent 0.5M ETT in Acetonitrile
BI0158

Activator Reagent 0.5M ETT in Acetonitrile

0.5M ETT in Acetonitrile

Oxidizing Reagent, .02M I<sub>2</sub>O in THF/Pyridine/H<sub>2</sub>O (70/20/10)
BI0420

Oxidizing Reagent, .02M I2O in THF/Pyridine/H2O (70/20/10)

Biosynthesis Novabiochem®

Capping Reagent A 20% n-Methylimidazole in Acetonitrile
BI0224

Capping Reagent A 20% n-Methylimidazole in Acetonitrile

Biosynthesis Novabiochem®

2-Cyanoethyl <I>N,N</I>-diisopropylchlorophosphoramidite
302309

2-Cyanoethyl N,N-diisopropylchlorophosphoramidite

Cl 13.5-15.5 %

Activator Reagent 0.3M BMT in AcN
BI0166

Activator Reagent 0.3M BMT in AcN

Biosynthesis Novabiochem®

<I>N</I><SUP>6</SUP>-Methyladenosine 5′-monophosphate sodium salt
M2780

N6-Methyladenosine 5′-monophosphate sodium salt

≥97% (HPLC)

Deblock Reagent 10% DCA in Toluene
BI0841

Deblock Reagent 10% DCA in Toluene

Biosynthesis Novabiochem®

2-Cyanoethyl <I>N</I>,<I>N</I>,<I>N</I>′,<I>N</I>′-tetraisopropylphosphorodiamidite
305995

2-Cyanoethyl N,N,N′,N′-tetraisopropylphosphorodiamidite

97%

Cytidine
C122106

Cytidine

99%

Capping Reagent B 16% n-Methylimidazole in THF
BI0345

Capping Reagent B 16% n-Methylimidazole in THF

Novabiochem®

Tetrazole solution
88185

Tetrazole solution

~0.45 M in acetonitrile, solution, suitable for DNA synthesis, filtered through a 1 μm filter

Capping Reagent B2 60% Lutidine in Acetonitrile
BI0349

Capping Reagent B2 60% Lutidine in Acetonitrile

Biosynthesis Novabiochem®

Capping Reagent B1 40% Acetic Anhydride in Acetonitrile
BI0347

Capping Reagent B1 40% Acetic Anhydride in Acetonitrile

Biosynthesis Novabiochem®

Deblocking Reagent 3% Trichloroacetic Acid in Dichloromethane
BI0830

Deblocking Reagent 3% Trichloroacetic Acid in Dichloromethane

Novabiochem®

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