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324699

Elastase Substrate IV, Colorimetric

A specific substrate for pancreatic elastase.

Synonym(s):

Elastase Substrate IV, Colorimetric

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Size/SKUAvailabilityPrice
5 mg
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$145.00

About This Item

Empirical Formula (Hill Notation):
C25H34N6O9
Molecular Weight:
562.57
NACRES:
NA.54
UNSPSC Code:
12352200
Assay:
≥95% (HPLC)
Form:
lyophilized solid
Storage condition:
OK to freeze, avoid repeated freeze/thaw cycles, desiccated

$145.00


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Quality Segment

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles, desiccated

color

white to off-white

solubility

ethanol: 1 mg/mL, water: soluble

shipped in

ambient

storage temp.

−20°C

General description

A specific substrate for pancreatic elastase (Km = 100 µM; Kcat/Km = 35,000 s-1 M-1 for rat pancreatic elastase; Km = 30 µM; Kcat/Km = 351,000 s-1 M-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.



Suggested protocol: see Largman, C. (1983)

Biochem/physiol Actions

Cell permeable: no
Primary Target
A specific substrate for pancreatic elastase
Product does not compete with ATP.
Reversible: no
km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine

Physical form

45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

Other Notes

Largman, L. 1983. Biochemistry22, 3763.
Del Mar, E.G., et al. 1980. Biochemistry19, 468.
Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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assay

≥95% (HPLC)

assay

≥95% (HPLC)

assay

≥95% (HPLC), ≥95% (TLC)

assay

≥98% (HPLC)

form

lyophilized solid

form

lyophilized

form

lyophilized solid, powder

form

lyophilized

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

OK to freeze, desiccated, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, desiccated

storage condition

OK to freeze, desiccated, protect from light

storage condition

OK to freeze, desiccated, protect from light


Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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