This product is not tested for suitability in any specific application. The material is intended for general laboratory research use. The purity value is listed on the lot-specific Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under ‘Certificate of Analysis’:
https://www.sigmaaldrich.com/product/mm/851209#product-documentation
8.51209
D(+)-Biotin
Novabiochem®
Synonym(s):
D(+)-Biotin, D-Biotin,Vitamin H, Hexahydro-2-oxothieno(3,4-d)-imidazole-4-pentanoic acid, Vitamin H
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| Size/SKU | Availability | Price |
|---|---|---|
1 g | Check Cart for Availability | $77.20 |
5 g | Check Cart for Availability | $278.00 |
25 g | Check Cart for Availability | $1,110.00 |
About This Item
Empirical Formula (Hill Notation):
C10H16N2O3S
CAS Number:
Molecular Weight:
244.31
UNSPSC Code:
41116133
EC Index Number:
200-399-3
NACRES:
NA.22
MDL number:
Quality Segment
product line
Novabiochem®
form
solid
potency
>2000 mg/kg LD50, oral (Rat)
reaction suitability
reaction type: Biotinylations
manufacturer/tradename
Novabiochem®
pH
4.5 (0.1 g/L in H2O)
mp
232-233 °C
solubility
0.2 g/L
bulk density
200‑500 kg/m3
application(s)
peptide synthesis
storage temp.
2-8°C
SMILES string
S1C(C2NC(=O)NC2C1)CCCCC(=O)O
InChI
1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)
InChI key
YBJHBAHKTGYVGT-UHFFFAOYSA-N
General description
D(+)-Biotin is the simplest reagent for the introduction of biotin to peptides, proteins and other biomolecules. In SPPS, it is best coupled using DIPCDI/Oxyma Pure. Biotin-labeled peptides have many important applications in immunology and histochemistry, such as affinity purification and FRET-based flow cytometry, solid-phase immunoassays, and receptor localization, that exploit the high affinity of streptavidin and avidin for biotin.,,,
Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis
Literature references:
[1] K. Hofmann & Y. Kiso (1976) Proc. Natl. Acad. Sci., 73, 3516.
[2] T. Buranda, et al. (1999) Cytometry, 37, 21.
[3] I. Sélo, et al. (1996) J. Immunol. Methods, 199, 127.
[4] J. Howl, et al. (1993) Eur J Biochem., 213, 711.
Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis
Literature references:
[1] K. Hofmann & Y. Kiso (1976) Proc. Natl. Acad. Sci., 73, 3516.
[2] T. Buranda, et al. (1999) Cytometry, 37, 21.
[3] I. Sélo, et al. (1996) J. Immunol. Methods, 199, 127.
[4] J. Howl, et al. (1993) Eur J Biochem., 213, 711.
Application
Some recent applications of D(+)-Biotin in SPPS:
- In the high-throughput biotinylation of proteins. The approach for site-specific biotinylation of proteins is useful for various applications such as protein purification and analysis.
- In the site-specific biotinylation of multipodal glycoclusters on a solid support. The synthesis of biotinylated glycoclusters, has important applications in glycobiology.
Legal Information
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| description Novabiochem® | description - | description Pharmaceutical Secondary Standard; Certified Reference Material | description certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland |
| form solid | form solid | form - | form - |
| application(s) peptide synthesis | application(s) - | application(s) pharmaceutical (small molecule) | application(s) clinical testing |
| solubility 0.2 g/L | solubility water: 1 mg/mL | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-30°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 100 | Quality Level 300 | Quality Level 300 |
| mp 232-233 °C | mp - | mp 231-233 °C (lit.) | mp 231-233 °C (lit.) |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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