CBA415
Synonym(e):
Viability Assay Kit
| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
Warenkorb auf Verfügbarkeit prüfen | CHF 816.00 |
fluorometric
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| detection method fluorometric | detection method fluorometric | detection method - | detection method chemiluminescent |
10 - Combustible liquids
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Below are the volume and concentration of the reagents of the kit CBA415 (Live Dead Cell Viability Assay Kit for 3D and 2D cell culture)
All the volumes are also on the vial labels for each component.
Vial 1: Green cap: Calcein AM in DMSO: 25ul 1,91mg/ml
Vial 2: Red Cap: Propidium Iodide in DMSO: 100ul 3mg/ml
Vial 3: Blue Cap: Hoechst 33342 in DMSO: 40ul 7,5mg/ml
Helpful?
Live/dead cell assays are ideally performed when cells are roughly 50% confluent to ensure cells are actively dividing and not inhibited by contact, allowing accurate measurement of proliferation, drug effects, or viability. Treatment at <50% confluency allows for proper evaluation of anti-proliferative effects without being affected by contact inhibition.
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As part of the protocol for CBA415, one must induce cytotoxicity. Therefore, we have not studied maintaining live organoids after using this kit. Please refer to the protocol for explanations of this kit's functions.
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The exact concentrations of Calcein-AM, PI, and Hoechst are proprietary and cannot be disclosed.
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Is it possible to use formaldehyde or formalin for fixing cells before utilizing this live/dead cell staining solution?
The use of this kit on formaldehyde or formalin fixed cells has not been assessed in our lab, and our literature survey has not provided a recommended protocol reference.
It's important to note that fixing cells with a crosslinker such as formalin or formaldehyde could potentially impact the results of this assay. The harsh conditions of crosslinking may lead to cell membrane damage and increased cell death. As this has not been tested in our lab, we highly recommend conducting a pilot experiment to confirm compatibility.
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Working solutions of the Propidium Iodide and the Hoechst 33342 are stable for at least 72 hours when refrigerated and protected from light. Calcein AM, once diluted to a working concentration in a aqueous diluent, will begin to degrade within several hours and storage is not recommended.
Helpful?
The dye solution for cell staining should be prepared just before use. Store at -20oC or below, and protect from light. The vials can be frozen and thawed up to 10 times. Before use, allow the vials to thaw to room temperature and centrifuge briefly.
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