We do not offer for this buffer separately, but we do have a related product that may help: Catalog number: FLAAB.
SCT149
ATP Cell Viability Luciferase Assay
A highly sensitive firefly luciferase cell based assay for quantifying ATP in cell cultures used to measure cell viability.
Synonym(s):
Luminescent Cell Viability Assay, Cell Toxcity Assay, Bioluminescent Cell Viability Assay
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $548.00 |
About This Item
NACRES:
NA.47
UNSPSC Code:
12352207
$548.00
Check Cart for Availability
technique(s)
cell based assay: suitable
detection method
chemiluminescent
shipped in
ambient
storage temp.
-65 to -96°C
General description
Because ATP is an indicator of metabolically active cells, the number of viable cells can be assessed based on the amount of ATP available. The ATP Cell Viability Luciferase Assay offers a highly sensitive homogenous assay for quantifying ATP in cell cultures. This kit takes advantage of Firefly luciferase’s use of ATP to oxidize D-Luciferin and the resulting production of light in order to assess the amount of ATP available in cell cultures. The sensitive assay procedure involves a single addition of ATP detection cocktail directly to cells cultured in a serum-supplemented medium. No cell washing, medium removal and multiple pipetting are required. The kit can be used to detect as little as a single cell or 0.01 picomoles of ATP. The signal produced is linear within 6 orders of magnitude. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from the determination of viable cell numbers to cell proliferation to cell cytotoxicity.
The ATP Cell Viability Luciferase Assay is a flash-type luminescence assay designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format. The luminescence signal generated is stable for about 1 minute.
The ATP Cell Viability Luciferase Assay is a flash-type luminescence assay designed for individual sample detection by using a luminometer in a single sample format or a luminometer with an injector in 96-well plate format. The luminescence signal generated is stable for about 1 minute.
Application
A highly sensitive firefly luciferase cell based assay for quantifying ATP in cell cultures used to measure cell viability.
Cell Viability Assay
Research Category
Cell Signaling
Cell Signaling
Research Sub Category
Live Cell Dye
Live Cell Dye
Physical form
Liquid
Preparation Note
Store ATP Cell Viability Luciferase Assay at -80°C. Avoid repeated freeze-thaw cycles. Product is stable for at least 6 months from date of receipt when stored as recommended.
Other Notes
1) D-Luciferin (CS224519): 4 X 10 mg
2) Firefly Luciferase (CS224520): 5 X 200 µL
3) ATP Assay Buffer (CS224521): 1 X 100 mL
4) ATP, 2µM (CS224522): 1 X 100 µL
2) Firefly Luciferase (CS224520): 5 X 200 µL
3) ATP Assay Buffer (CS224521): 1 X 100 mL
4) ATP, 2µM (CS224522): 1 X 100 µL
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description A highly sensitive firefly luciferase cell based assay for quantifying ATP in cell cultures used to measure cell viability. | description for cellular ATP determination | description Glow-type luciferase assay for the quantification of firefly luciferase gene expression in mammalian cells with signal half-life of about 3 hours | description This proliferation assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells. |
| detection method chemiluminescent | detection method - | detection method chemiluminescent | detection method colorimetric |
| technique(s) cell based assay: suitable | technique(s) - | technique(s) cell based assay: suitable | technique(s) - |
| storage temp. -65 to -96°C | storage temp. −20°C | storage temp. -65 to -96°C | storage temp. −20°C |
| shipped in ambient | shipped in - | shipped in ambient | shipped in wet ice |
Storage Class
10 - Combustible liquids
wgk
WGK 3
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Can the buffer used in this kit be made or is it sold separately? It seems like that component of the kit will run out before the rest of the components.
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