EZIL6
Human IL-6 ELISA Kit
detect IL-6 in 50 μL serum, plasma or cell culture supernatent
Synonym(s):
B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, IL6, Interferon beta-2, Interleukin-6
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
1 pkg | Check Cart for Availability | $723.00 |
5 x 1 pkg | Check Cart for Availability | $3,310.00 |
10 x 1 pkg | Check Cart for Availability | $6,010.00 |
About This Item
sample type plasma (K2 EDTA)
sample type cell culture supernatant
Product Name
Human IL-6 Conferma® ELISA, quantification of human IL-6 in serum, plasma, or culture media samples
Quality Segment
species reactivity
human
parameter
50 μL sample volume (4hr assay)
technique(s)
ELISA: suitable
input
sample type serum
sample type plasma (K2 EDTA)
sample type cell culture supernatant
UniProt accession no.
application(s)
research use
assay range
linearity: 105-116%
(serum), linearity: 88-105%
(plasma), inter-assay cv: <15%
intra-assay cv: <5%
standard curve range: 1.17-300 pg/mL
accuracy: 91-109%
detection method
colorimetric (450nm/590nm)
storage temp.
2-8°C
Gene Information
human ... IL6(3569)
General description
This is a 96 well format sandwich ELISA which uses our Conferma® process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots. For detailed information on the process and a Verification report for this assay please click hereConferma™ Sandwich ELISAs
Entrez Gene Summary:
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections.
UniProt Summary:
- Function: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism.
- Size: 212 amino acids; 23,718 Da
- Subunit: Component of a hexamer of two molecules each of IL6, IL6R and IL6ST.
- Subcellular location: Extracellular region or secreted
- Disease: Rheumatoid arthritis systemic juvenile (RASJ). A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
- Similarity: Belongs to the IL-6 superfamily.
Application
Research Category:
- Cytokines
- Inflammation & Immunology
- Immune Response
- Metabolic Disease
Analysis Note
Legal Information
Disclaimer
1 of 1
This Item | |||
|---|---|---|---|
| description quantification of human IL-6 in serum, plasma, or culture media samples | description quantification of human MCP-1 in serum, plasma, or culture media samples. | description for cell and tissue lysates | description for serum, plasma and cell culture supernatant |
| Gene Information human ... IL6(3569) | Gene Information human ... CCL2(6347) | Gene Information mouse ... Il6(24498) | Gene Information mouse ... Il6(24498) |
| species reactivity human | species reactivity human | species reactivity mouse | species reactivity mouse |
| assay range linearity: 105-116% | assay range linearity: 94-110 (plasma)%, inter-assay cv: <12.9% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% |
| input sample type serum | input sample type plasma (K2 EDTA) | input sample type tissue lysate | input sample type plasma |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level - |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) capture ELISA: suitable |
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