MAB8887
Anti-Collagen Type II (COL2A1) Antibody
CHEMICON®, mouse monoclonal, 6B3
Synonym(s):
Anti-Anti-ANFH, Anti-Anti-AOM, Anti-Anti-COL11A3, Anti-Anti-SEDC, Anti-Anti-STL1
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
50 μg | Check Cart for Availability | $508.00 |
About This Item
Product Name
Anti-Collagen Type II Antibody, clone 6B3, clone 6B3, Chemicon®, from mouse
biological source
mouse
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6B3, monoclonal
species reactivity
human, chicken, mouse, salamander
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... COL2A1(1280)
General description
Immunogen
Application
Cell Structure
ECM Proteins
Immunohistochemistry (Formalin/Paraffin): 1-2 μg/mL, 30 minutes at room temperature. Staining of paraffin embedded tissues requires digestion of tissue sections with pepsin at 1mg/mL in Tris HCl, pH 2.0 for 15 minutes at RT or 10 minutes at 37ºC.
Immunofluorescence
ELISA
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Its epitope is localized in the triple helix of type II collagen. It shows no cross-reaction with type I or type III collagen. Immunoblotting of CNBr peptides of collagen II shows that MAB8887 reacts with CB11 (25kDa) fragment which is the site of immunogenic and arthritogenic epitopes along the intact type II molecule.
In pepsin solublized collagen II, MAB8887 reacts with a 95-97 kDa fragment, as well as, the native single chain of 120kDa. If propeptides are present, MAB8887 will detect a 200kDa fragment.
Physical form
Preparation Note
Analysis Note
Cartilage in lung or fetus
Other Notes
Legal Information
Disclaimer
1 of 1
This Item | |||
|---|---|---|---|
| description clone 6B3, Chemicon®, from mouse | description Chemicon®, from rabbit | description affinity isolated antibody | description recombinant, expressed in HEK 293 cells |
| biological source mouse | biological source rabbit | biological source rabbit | biological source rabbit |
| species reactivity human, chicken, mouse, salamander | species reactivity human | species reactivity human, mouse, rat | species reactivity human, chicken |
| antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form purified antibody |
| clone 6B3, monoclonal | clone polyclonal | clone polyclonal | clone 1K11, recombinant monoclonal |
| Gene Information human ... COL2A1(1280) | Gene Information human ... COL2A1(1280) | Gene Information human ... COL2A1(1280) | Gene Information human ... COL2A1(1280) |
| technique(s) western blot: suitable, immunofluorescence: suitable, immunohistochemistry: suitable | technique(s) immunohistochemistry: suitable, ELISA: suitable, immunocytochemistry: suitable, western blot: suitable | technique(s) ELISA: 1:20000, western blot: 1:500-1:1000, immunohistochemistry: 1:50-1:100, immunofluorescence: 1:100-1:500 | technique(s) western blot: suitable, immunohistochemistry: suitable, immunocytochemistry: suitable, ELISA: suitable, flow cytometry: suitable |
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
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