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S7807

CpG WIZ Fragile X Amplification Kit

The components of the CpG WIZ Fragile X Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.

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25 assays
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$820.00

About This Item

UNSPSC Code:
12161503
NACRES:
NA.31
eCl@ss:
32161000

$820.00


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Quality Segment

species reactivity

human

manufacturer/tradename

CPGWIZ, Chemicon®

technique(s)

PCR: suitable

NCBI accession no.

UniProt accession no.

application(s)

genomic analysis

shipped in

dry ice

Gene Information

human ... FRAXA(2477)

General description

Methylation of cytosines located 5′ to guanosine is known to have a profound effect on the expression of several eukaryotic genes (Bird, 1992). In normal cells, methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG islands, remain unmethylated. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells (Antequera, 1990) and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers (Herman, 1994; Merlo, 1995). The Fragile X gene exhibits characteristic hypermethylation.



Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.

Application

Principles of the Technique

Use of either the CpGenome DNA Modification Kit (Cat. No. S7820) or the CpGENOME Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the CpG WIZ Fragile X Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.
The components of the CpG WIZ Fragile X Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.

Other Notes

The components of the CpG WIZ Fragile X Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples with appropriate controls.

U Primer Set5 μM each primer (25X) 35 μL (neurtal cap) 90519 -15°C to -25°C

M Primer Set5 μM each primer (25X) 35 μL (red cap) 90520 -15°C to -25°C

W Primer Set5 μM each primer (25X) 35 μL (green cap) 90521 -15°C to -25°C

U control DNA0.1 μg/μL 50 μL (white cap) 90393 -15°C to -25°C

M control DNA0.1 μg/μL 50 μL (red cap) 90394 -15°C to -25°C

W control DNA0.05 μg/μL 50 μL (green cap) 90395 -15°C to -25°C

Universal 10X PCR Buffer 265 μL (blue cap) 90396 -15°C to -25°C

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
CPGWIZ is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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description

The components of the CpG WIZ Fragile X Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.

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The components of the CpG WIZ GSTpi Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.

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The components of the CpG WIZ H19-IGF2 Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples.

description

The components of the CpG WIZ MGMT Amplification Kit include those required for PCR amplification after bisulfite modification of DNA samples. Sufficient reagents are provided to analyze 25 samples.

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

application(s)

genomic analysis

application(s)

genomic analysis

application(s)

genomic analysis

application(s)

genomic analysis

NCBI accession no.

NM_002024.3

NCBI accession no.

NM_000852.2

NCBI accession no.

NM_001007139.3, NM_000612.3

NCBI accession no.

NM_002412.2

UniProt accession no.

Q06787

UniProt accession no.

-

UniProt accession no.

P01344

UniProt accession no.

-

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

manufacturer/tradename

Chemicon®, CPGWIZ

manufacturer/tradename

CPGWIZ, Chemicon®

manufacturer/tradename

CPGWIZ, Chemicon®

manufacturer/tradename

Chemicon®, CPGWIZ


Storage Class

10 - Combustible liquids



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