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SF016

ESGRO-2i Medium, 100ml

to enhance viability of mouse embryonic stem and iPS cells

Synonym(s):

2i Naive ES/IPS Media

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100 mL
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$188.00
200 mL
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$313.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.81
eCl@ss:
32160801

$188.00

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Quality Segment

100

form

liquid

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

General description

Two stages of mouse pluripotency have been stabilized to date through in vitro culture. ‘Naive’ represents the earliest stage, which can be enriched for a purer population, termed ‘ground state’. Naive most closely approximates embryonic epiblast in the preimplantation blastocyst. The primed state most closely approximates in vivo immediate postimplantation epiblast. There are clear differences between these stages in culture, expression, epigenetics (including appropriate X-inactivation) and developmental competence. The fact that naive mESCs can generate whole animals through tetraploid complementation while primed mESCs cannot, is a particularly compelling reason for heightened expectations from naive hESCs.
ESGRO-2i medium is a defined, LIF containing medium provided with a selective GSK3β and Mek 1/2 inhibitors to enhance viability of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for cell expansion and growth at clonal density in serum-free conditions and supports a naive/ground pluripotent state.

Application

Suitable for ES and iPS cell derivation, passaging and maintenance. Media can be used on feeder dependent and independent pluirpotent stem cell lines.

Important passaging information
Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
Recommended products include:
ESGRO Complete™ Accutase (Cat # SF006)
ESGRO Complete Trypsin Solution (proprietary) (Cat # SF007)
ESGRO Complete Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)

Preparation Note

• Upon receipt, store basal medium and inhibitor supplements in the dark at -20°C until use. Refer to lot expiration date on labels.
• After basal medium and inhibitor supplements are combined, store the complete ESGRO-2i medium in the dark at 4°C for up to 10 days. Do not refreeze.
1. ESGRO-2i Basal Medium with LIF: (Catalogue No. CS204463) One (1) 100 mL bottle containing basal medium supplemented with LIF, pH 7.1-7.3.

2. GSK3β Inhibitor Supplement: (Catalogue No. SF012-050) One (1) 50 μL vial containing selective GSK3β inhibitor supplement.

3. Mek1/2 Inhibitor Supplement: (Catalogue No. CS204465) One (1) 10 μL vial containing selective Mek 1/2 inhibitor supplement.

Legal Information

cOmplete is a trademark of Roche

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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ESGRO-2i Medium, 100ml to enhance viability of mouse embryonic stem and iPS cells

Sigma-Aldrich

SF016

ESGRO-2i Medium, 100ml

ESGRO®-2i Supplement Kit (1000x) enhances viability of mouse embryonic stem and iPS cells in the absence of serum & feeder cells

Sigma-Aldrich

ESG112

ESGRO®-2i Supplement Kit (1000x)

ESGRO Complete PLUS Clonal Grade Medium, 100ml to enhance viability of mouse embryonic stem cells

Sigma-Aldrich

SF001

ESGRO Complete PLUS Clonal Grade Medium, 100ml

ESGRO Complete Basal Medium, 100mL The ESGRO Complete Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF & BMP.

Sigma-Aldrich

SF002

ESGRO Complete Basal Medium, 100mL

description

to enhance viability of mouse embryonic stem and iPS cells

description

enhances viability of mouse embryonic stem and iPS cells in the absence of serum & feeder cells

description

to enhance viability of mouse embryonic stem cells

description

The ESGRO Complete Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF & BMP.

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

form

liquid

form

liquid

form

liquid

form

liquid

Quality Level

100

Quality Level

100

Quality Level

100, 300

Quality Level

300

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

Storage Class

10 - Combustible liquids

wgk

WGK 2

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Questions

  1. ESGRO-2i Medium. So, MEK inhibitor, PD0325901, is contained with this kit, right?

    1 answer

    1. An MEK inhibitor is included in this kit. This product is supplied with the following components:
      ESGRO-2i Basal Medium with LIF: (Catalogue No. CS204463) One (1) 100 mL bottle containing basal medium supplemented with LIF, pH 7.1-7.3.
      2. GSK3β Inhibitor Supplement: (Catalogue No. SF012-050) One (1) 50 μL vial containing selective GSK3β inhibitor supplement.
      3. Mek1/2 Inhibitor Supplement: (Catalogue No. CS204465) One (1) 10 μL vial containing selective Mek 1/2 inhibitor supplement.

      More information can be viewed on the following Information Sheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/526/239/sf016-100.pdf

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