The Amicon Ultra 0.5 products are similar in size to a 1.5 mL Eppendorf tube. Refer to the User Guide for the dimensions. It is recommended to spin in a centrifuge below the maximum speed of 14,000 x g. Conversion to x g should be done either by calculation or by using an electronic switch on the centrifuge, rather than using r.p.m. for speed.
UFC5050
Amicon® Ultra Centrifugal Filter, 50 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 50 kDa
Sustainability features
Synonym(s):
Centrifugal concentrator, 50 kDa MWCO, 0.5 mL sample volume
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| Size/SKU | Availability | Price |
|---|---|---|
24 pkg | Estimated to ship onOctober 19, 2026 | $632.00 |
96 pkg | Estimated to ship onOctober 19, 2026 | $632.00 |
500 pkg | Estimated to ship onOctober 19, 2026 | $2,580.00 |
About This Item
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
Pore size:
50 kDa MWCO
Working volume:
≤500 μL , 0.5 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg
Quality Segment
material
polypropylene cap, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 500 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Sustainability features
parameter
0.5 mL sample volume
technique(s)
Diafiltration: suitable, protein extraction: suitable, protein purification: suitable
L
4.99 cm (1.96 in.)
diam.
10.8 mm
filtration area
1 cm2
volume
0.5 mL
working volume
≤500 μL , 0.5 mL
pore size
50 kDa MWCO
shipped in
ambient
General description
Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, BSA (67 kDa) at 1 mg/ml initial concentration is typically retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 50,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
Features and Benefits
- Ultracel® regenerated cellulose membrane with 50 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
Physical form
Color Code: Clear
Other Notes
Replaces: 42415
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| description sample volume 0.5 mL, regenerated cellulose membrane, MWCO 50 kDa | description sample volume 4 mL, regenerated cellulose membrane, MWCO 50 kDa | description sample volume 2 mL, regenerated cellulose membrane, MWCO 50 kDa | description sample volume 15 mL, regenerated cellulose membrane, MWCO 50 kDa |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| pore size 50 kDa MWCO | pore size 50 kDa MWCO | pore size 50 kDa MWCO | pore size 50 kDa MWCO |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| parameter 0.5 mL sample volume | parameter 4 mL sample volume | parameter 2 mL sample volume | parameter 15 mL sample volume |
| material styrene-butadiene housing, polypropylene cap, regenerated cellulose membrane, polypropylene tube | material polyethylene liner, polypropylene tube, regenerated cellulose membrane, styrene-butadiene housing, polyethylene cap, regenerated cellulose support | material styrene-butadiene housing, regenerated cellulose membrane, polypropylene tube (for collection) | material polyethylene liner, styrene-butadiene housing, polyethylene cap, regenerated cellulose membrane, polypropylene tube, regenerated cellulose support |
| working volume 0.5 mL , ≤500 μL | working volume ≤4 mL | working volume 2 mL , ≤2 mL | working volume 15 mL , ≤15 mL |
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Is it compatible with the microcentrifuge machine (max 20000 r.p.m.) for 1.5 mL eppendorf tubes?
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UFC505096について、耐用gを教えてください。
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This product is not assigned an expiration date but end users should check stock after 3 years. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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