It is possible to spin this product at a lower speed and still achieve a decent flow rate.
UFC8050
Amicon® Ultra Centrifugal Filter, 50 kDa MWCO
sample volume 4 mL, regenerated cellulose membrane, MWCO 50 kDa
Sustainability features
Synonym(s):
Centrifugal concentrator, 50 kDa MWCO, 4 mL sample volume
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| Size/SKU | Availability | Price |
|---|---|---|
8 pkg | Check Cart for Availability | $82.30 |
24 pkg | Check Cart for Availability | $210.00 |
96 pkg | Check Cart for Availability | $755.00 |
About This Item
UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
Pore size:
50 kDa MWCO
Working volume:
≤4 mL , 4 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
Quality Segment
material
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Sustainability features
parameter
4 mL sample volume
technique(s)
Diafiltration: suitable, protein extraction: suitable, protein purification: suitable
L
124 mm (4.88 in.)
diam.
17.3 mm
filtration area
3 cm2
volume
4 mL
working volume
≤4 mL , 4 mL
pore size
50 kDa MWCO
shipped in
ambient
General description
Amicon® Ultra 4 mL centrifugal devices with Ultracel® 50,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example,BSA (67 kDa) at 1 mg/ml initial concentration is retained at >95% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.
The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.
Application
- Ultrafiltration with 50,000 Dalton molecular weight cutoff Ultracel® membrane.
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
- Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC.
- Concentration of dilute or pre-purified proteins from column eluents.
- Desalting, buffer exchange and protein dialysis.
- Size exclusion and protein fractionation.
Features and Benefits
- Ultracel® regenerated cellulose membrane with 50 kDa NMWCO delivers retentate recovery of greater than 90%.
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes).
- Heat-sealed membrane minimizes downstream extractables.
- 100% integrity tested for reliable performance.
- Convenient sample monitoring with translucent housing and volume gradations.
- Direct pipette sample access eliminates processing step to recover concentrate.
- High concentration factors of 80–100X.
Physical form
Color Code: Clear with pink cap
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| description sample volume 4 mL, regenerated cellulose membrane, MWCO 50 kDa | description sample volume 0.5 mL, regenerated cellulose membrane, MWCO 50 kDa | description sample volume 15 mL, regenerated cellulose membrane, MWCO 50 kDa | description sample volume 2 mL, regenerated cellulose membrane, MWCO 50 kDa |
| parameter 4 mL sample volume | parameter 0.5 mL sample volume | parameter 15 mL sample volume | parameter 2 mL sample volume |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| pore size 50 kDa MWCO | pore size 50 kDa MWCO | pore size 50 kDa MWCO | pore size 50 kDa MWCO |
| material styrene-butadiene housing, regenerated cellulose support, polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane | material regenerated cellulose membrane, polypropylene tube, polypropylene cap, styrene-butadiene housing | material regenerated cellulose support, polyethylene cap, polyethylene liner, regenerated cellulose membrane, styrene-butadiene housing, polypropylene tube | material styrene-butadiene housing, regenerated cellulose membrane, polypropylene tube (for collection) |
| working volume 4 mL , ≤4 mL | working volume ≤500 μL , 0.5 mL | working volume 15 mL , ≤15 mL | working volume ≤2 mL |
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If I cannot reach the suggested speed for this (4000xg for swingout, 7500xg for fixed-angle rotor), does this product still work at lower speeds?
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