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| Size/SKU | Availability | Price |
|---|---|---|
50 μL | Available to ship TODAYfromMILWAUKEE | $557.00 |
About This Item
Empirical Formula (Hill Notation):
C32H52N7O18P3S
CAS Number:
Molecular Weight:
947.78
UNSPSC Code:
41116100
MDL number:
NACRES:
NA.54
Form:
solution
Solubility:
water: miscible
Storage temp.:
−20°C
$557.00
Available to ship TODAYDetails
form
solution
packaging
pkg of 50 μL (50 nmol; 1mM)
manufacturer/tradename
Roche
color
colorless
solubility
water: miscible
storage temp.
−20°C
General description
Biotin is a water-soluble vitamin which functions as a coenzyme for carboxylases.Biotin-16-dUTP is used to produce biotinylated DNA probes that are commonly used to detect specific DNA and RNA sequences in different methods like Southern and Northern blotting, in situ hybridization, and sandwich assays.
Application
Biotin-16-dUTP has been used:
- For nonradioactive DNA labeling such as random priming, PCR labeling, or nick translation. Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
- as a substrate for terminal transferase, DNA polymerase I (holoenzyme and Klenow fragment), Taq DNA polymerase, and reverse transcriptase (e.g., Transcriptor). The nucleotide also serves as a substrate for Terminal Transferase in 3′-end labeling
- In fluorescence in situ hybridization (FISH)
Biochem/physiol Actions
Biotin influences gene regulation and chromatin structure by associating with lysine residues in histones. It influences the actions of adaptive immune T and natural killer cells. Deficiency of biotin in mice has indicated enhanced inflammation. Studies have also indicated heightened inflammatory response of human dendritic cells in conditions of biotin deficiency.
Analysis Note
Typical analysis: At least 85% deoxynucleoside triphosphate (Biotin-16-dUTP) (HPLC, area%).
Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial).
Notes: For your convenience, biotin-dUTP is also available in convenient labeling mixes (single solution with conventional dNTPs and biotin-16-dUTP), and technique-specific mixes (nucleotides, buffers, and enzymes in a single vial).
Absorption: The spectral data of Biotin-16-dUTP (at pH 7.3):
Emax241: E = 10.700;
Emax292: E = 7.000;
Emax265: E = 4.400;
A292/265 : 1.6.
Emax241: E = 10.700;
Emax292: E = 7.000;
Emax265: E = 4.400;
A292/265 : 1.6.
Other Notes
Biotin-labeled DNA can be detected with: Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate- Biotin Luminescence Detection Kit.
1 of 1
This Item | |||
|---|---|---|---|
| description - | description - | description =98%, solution, 100 mM, suitable for PCR, suitable for DNA sequencing | description Lithium salt |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche |
| form solution | form solution | form solution | form solution |
| solubility water: miscible | solubility water: miscible | solubility - | solubility - |
| color colorless | color clear red | color - | color - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| packaging pkg of 50 μL (50 nmol; 1mM) | packaging pkg of 25 μL (25 nmol; 1mM) | packaging pkg of 250 μL (25 μmol; 100 mM) | packaging pkg of 250 μL (25 μmol) |
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
does not flash
flash_point_c
does not flash
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