Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
50 μg | Available to ship TODAYfromMILWAUKEE | $208.00 |
About This Item
UNSPSC Code:
41105335
NACRES:
NA.54
Form:
solution
Solubility:
water: miscible
Storage temp.:
−20°C
$208.00
Available to ship TODAYDetails
form
solution
packaging
pkg of 50 μg (250 μg/ml)
manufacturer/tradename
Roche
concentration
250 μg/mL
color
colorless
solubility
water: miscible
storage temp.
−20°C
General description
Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 50 to 750 bp and an additional band of 2642 bp
Size Range: 50 to 750 bp and an additional band of 2642 bp
Application
DNA Molecular Weight Marker XIII (50 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest, followed by separation on agarose gels. DNA Molecular Weight Marker XIII (50 bp ladder) can be used in conjunction with DNA Molecular Weight Marker XIV (100 bp ladder) for precise size determination.
The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
- Restriction digests
- PCR
- RT-PCR
Physical form
Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0).
Preparation Note
Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.
Once thawed we recommend further storage at 2 to 8 °C.
Other Notes
50 μg = 1A260 unit
For life science research only. Not for use in diagnostic procedures.
The mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, and an additional band of 2642 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The 250- and 500-bp banding pattern is two to three times brighter.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The 250- and 500-bp banding pattern is two to three times brighter.
1 of 1
This Item | |||
|---|---|---|---|
| description pkg of 50 μg (250 μg/ml), solution | description solution, pkg of 50 μg (in 200 μl) | description solution, pkg of 50 μg (in 200 μl) | description pkg of 50 μg (in 200 μl), solution |
| manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche | manufacturer/tradename Roche |
| form solution | form solution | form solution | form solution |
| solubility water: miscible | solubility water: miscible | solubility water: miscible | solubility water: miscible |
| concentration 250 μg/mL | concentration 250 μg/mL | concentration 250 μg/mL | concentration 250 μg/mL |
| color colorless | color colorless | color colorless | color colorless |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
does not flash
flash_point_c
does not flash
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.