Skip to Content
MilliporeSigma

Skip To

EAGLETMMRO

Roche

EagleTaq Universal Master Mix (ROX)

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
500 x 20 reactions
Check Cart for Availability
$432.00

About This Item

NACRES:
NA.55
UNSPSC Code:
41106300

$432.00


Check Cart for Availability

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


usage

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions

feature

dNTPs included, hotstart

manufacturer/tradename

Roche

packaging

pkg of 100 x 20 μL reactions (07260288190), pkg of 500 x 20 μL reactions (07260296190), pkg of 5000 x 20 μL reactions (07249926190)

technique(s)

RT-qPCR: suitable, qPCR: suitable, whole genome amplification: suitable

input

purified DNA

detection method

probe-based

General description

2× concentrated, ready-to-use hot start master mix for qPCR and qRT-PCR using the hydrolysis probe detection format on real-time PCR instruments.

The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated PCR master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR hydrolysis probe reactions. It contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
The EagleTaq Universal Master Mix (ROX) contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
The hot start properties allow reaction setup at ambient temperature.
With this robust reagent, any PCR protocol optimization is minimized
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated polymerase chain reaction (PCR) master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR (qPCR) and quantitative reverse transcription PCR (qRT-PCR) hydrolysis probe reactions.

Application

EagleTaq Universal Master Mix (ROX) has been used in quantitative, real-time polymerase chain reaction (qPCR ) and quantitative reverse transcription PCR (qRT-PCR).

Features and Benefits

  • The EagleTaq Universal Master Mix (ROX) contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
  • It contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
  • The hot start properties allow reaction setup at ambient temperature.
  • With this robust reagent, any PCR protocol optimization is minimized.

Other Notes

Contains EagleTaq DNA Polymerase and a reaction buffer with dATP, dCTP, dGTP, dUTP, MgCl2, and ROX.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
KCQS09P4600FSTMPMMRO
description

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

description

with ROX for ABI instruments

description

with MgCl2

description

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 2000 reactions, suitable for qPCR, suitable for RT-qPCR

technique(s)

RT-qPCR: suitable

technique(s)

RT-qPCR: suitable

technique(s)

PCR: suitable

technique(s)

RT-qPCR: suitable

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart

feature

dNTPs included, hotstart: no

feature

dNTPs included: no, hotstart

usage

sufficient for 100 reactions, sufficient for 5000 reactions, sufficient for 500 reactions

usage

sufficient for 500 reactions

usage

sufficient for 100 reactions

usage

sufficient for 100 reactions, sufficient for 2000 reactions, sufficient for 500 reactions

packaging

pkg of 100 x 20 μL reactions (07260288190), pkg of 5000 x 20 μL reactions (07249926190), pkg of 500 x 20 μL reactions (07260296190)

packaging

-

packaging

-

packaging

pkg of 100 x 50 μL reactions (04673409001), pkg of 2000 x 50 μL reactions (04673433001), pkg of 500 x 50 μL reactions (04673417001)

detection method

probe-based

detection method

probe-based

detection method

-

detection method

probe-based

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche


Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library