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Roche

EagleTaq Universal Master Mix (ROX)

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

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Size/SKUAvailabilityPrice
500 x 20 reactions

Available to ship TODAYfromMILWAUKEE

$453.00

About This Item

NACRES:
NA.55
UNSPSC Code:
41106300

$453.00


Available to ship TODAYDetails


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usage

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions

feature

dNTPs included, hotstart

manufacturer/tradename

Roche

packaging

pkg of 100 x 20 μL reactions (07260288190), pkg of 500 x 20 μL reactions (07260296190), pkg of 5000 x 20 μL reactions (07249926190)

technique(s)

RT-qPCR: suitable, qPCR: suitable, whole genome amplification: suitable

input

purified DNA

detection method

probe-based

General description

2× concentrated, ready-to-use hot start master mix for qPCR and qRT-PCR using the hydrolysis probe detection format on real-time PCR instruments.

The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated PCR master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR hydrolysis probe reactions. It contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
The EagleTaq Universal Master Mix (ROX) contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
The hot start properties allow reaction setup at ambient temperature.
With this robust reagent, any PCR protocol optimization is minimized
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated polymerase chain reaction (PCR) master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR (qPCR) and quantitative reverse transcription PCR (qRT-PCR) hydrolysis probe reactions.

Application

EagleTaq Universal Master Mix (ROX) has been used in quantitative, real-time polymerase chain reaction (qPCR ) and quantitative reverse transcription PCR (qRT-PCR).

Features and Benefits

  • The EagleTaq Universal Master Mix (ROX) contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
  • It contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
  • The hot start properties allow reaction setup at ambient temperature.
  • With this robust reagent, any PCR protocol optimization is minimized.

Other Notes

Contains EagleTaq DNA Polymerase and a reaction buffer with dATP, dCTP, dGTP, dUTP, MgCl2, and ROX.

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FSUPMMROFSUSGMMRO05893151001
description

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

description

sufficient for 250 reactions, sufficient for 1250 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

description

-

description

sufficient for 100 reactions, suitable for cDNA synthesis, suitable for RT-PCR, suitable for RT-qPCR

technique(s)

RT-qPCR: suitable, whole genome amplification: suitable

technique(s)

whole genome amplification: suitable, qPCR: suitable, RT-qPCR: suitable

technique(s)

qPCR: suitable, whole genome amplification: suitable, RT-qPCR: suitable

technique(s)

RT-qPCR: suitable, cDNA synthesis: suitable, RT-PCR: suitable

usage

sufficient for 500 reactions, sufficient for 100 reactions, sufficient for 5000 reactions

usage

sufficient for 1250 reactions, sufficient for 5000 reactions, sufficient for 250 reactions

usage

sufficient for 200 reactions, sufficient for 2000 reactions

usage

sufficient for 100 reactions

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

detection method

probe-based

detection method

probe-based

detection method

probe-based

detection method

probe-based

input

purified DNA

input

purified DNA

input

purified DNA

input

purified RNA

feature

dNTPs included, hotstart

feature

dNTPs included: no, hotstart

feature

hotstart, dNTPs included: no

feature

dNTPs included: no, hotstart: no


Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash



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