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FORMD-RO

Roche

Formate Dehydrogenase

from Candida boidinii

Synonym(s):

FDH, Formate:NAD oxidoreductase

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80 UNITS

$309.00

250 UNITS

$554.00

$309.00

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About This Item

UNSPSC Code:
12352204
EC Number:
1.2.1.2 (BRENDA, IUBMB)
NACRES:
NA.54
Specific activity:
~0.4 units/mg protein (At 25 °C with formate as the substrate.)
Biological source:
yeast (Candida boidini)
Recombinant:
expressed in E. coli
Concentration:
≥10-20 % (w/w)

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biological source

yeast (Candida boidini)

recombinant

expressed in E. coli

form

lyophilized

specific activity

~0.4 units/mg protein (At 25 °C with formate as the substrate.)

mol wt

74 kDa

packaging

pkg of 250 U (10837016001), pkg of 80 U (10244678001)

manufacturer/tradename

Roche

concentration

≥10-20 % (w/w)

technique(s)

activity assay: suitable

color

off-white

optimum pH

7.5-8.5

solubility

water: soluble 10 g/L

suitability

suitable for gel electrophoresis

UniProt accession no.

O13437

application(s)

life science and biopharma

foreign activity

ADH <0.0500%, LDH <0.0500%, MDH <0.1000%

storage temp.

2-8°C

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This Item
F8649LMDH-ROM1567
Formate Dehydrogenase from Candida boidinii

Roche

FORMD-RO

Formate Dehydrogenase

Formate Dehydrogenase from Candida boidinii lyophilized powder, 5.0-15.0&#160;units/mg protein

Sigma-Aldrich

F8649

Formate Dehydrogenase from Candida boidinii

L-Malate Dehydrogenase (L-MDH) from pig heart (mitochondrial)

Roche

LMDH-RO

L-Malate Dehydrogenase (L-MDH)

Malic Dehydrogenase from porcine heart &#8805;600&#160;units/mg protein (biuret), ammonium sulfate suspension

Sigma-Aldrich

M1567

Malic Dehydrogenase from porcine heart

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

activity assay: suitable

technique(s)

-

specific activity

~0.4 units/mg protein (At 25 °C with formate as the substrate.)

specific activity

5.0-15.0 units/mg protein

specific activity

~1200 units/mg protein (At 25 °C with oxaloacetate as the substrate.)

specific activity

≥600 units/mg protein (biuret)

biological source

yeast (Candida boidini)

biological source

fungus (Candida boidinii)

biological source

Porcine heart (mitochondrial)

biological source

-

form

lyophilized

form

lyophilized powder

form

solution, suspension

form

ammonium sulfate suspension

concentration

≥10-20 % (w/w)

concentration

-

concentration

-

concentration

-

suitability

suitable for gel electrophoresis

suitability

-

suitability

suitable for Western blot

suitability

-

General description

Formate:NAD+ oxidoreductase
Formate dehydrogenase is part of the D-specific 2-hydroxy acid superfamily of dehydrogenases. It is a dimer of two identical subunits. The enzyme has a cysteine residue which is essential for its activity or structural integrity.[1]

Application

Most widely used in cofactor recycling systems for NADH.[2]

Biochem/physiol Actions

Substrate Specificity and Km:
Formate dehydrogenase reacts only with formate (Km = 13 mM at 30 °C and pH 7.5; 11 mM at 25 °C and pH 7.5) and NAD (Km 0.09 mM; 30 °C; pH 7.5). It does not react with acetate, oxalate, lactate, succinate, propionate or ascorbate, nor will the enzyme reduce NADP.

Preparation Note

Store dry

Analysis Note

Contaminants: <0.005% LDH and ADH each, <0.1% MDH

Other Notes

For life science research only. Not for use in diagnostic procedures.
One unit (U) formate dehydrogenase will oxidize 1 μmol of formic acid to CO2 in 1 minute at +25 °C and pH 7.6. The above assay produces 1 mmol of NADH per mmol of formate oxidized.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
70470222
63834423
62523520
46793423
46793420

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Katja Schirwitz et al.
Protein science : a publication of the Protein Society, 16(6), 1146-1156 (2007-05-26)
The understanding of the mechanism of enzymatic recovery of NADH is of biological and of considerable biotechnological interest, since the essential, but expensive, cofactor NADH is exhausted in asymmetric hydrogenation processes, but can be recovered by NAD(+)-dependent formate dehydrogenase (FDH).
Knut Erik Hovda et al.
Scandinavian journal of clinical and laboratory investigation, 75(7), 610-614 (2015-07-24)
The standard diagnostic approach to methanol poisoning is chromatographic measurement of methanol on centrally placed stationary equipment. Methanol poisoning in places where such equipment is unavailable is thus often not diagnosed. Methanol is metabolized to a toxic metabolite, formate; the

Global Trade Item Number

SKUGTIN
1024467800104061838669636
1083701600104061838683885

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