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ROAMYGLL

Roche

Amyloglucosidase

from Aspergillus niger

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Synonym(s):

Aspergillus niger, disaccharidase-type a-glucosidase

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500 units

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$203.00
3500 units
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$600.00

About This Item

UNSPSC Code:
12352204
EC Number:
NACRES:
NA.54
Specific activity:
6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate), ~6 units/mg protein (At 25 °C with glycogen as the substrate.)
Biological source:
Aspergillus niger

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biological source

Aspergillus niger

form

lyophilized

specific activity

6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate), ~6 units/mg protein (At 25 °C with glycogen as the substrate.)

mol wt

97 kDa

packaging

pkg of 3,500 U (11202367001), pkg of 500 U (11202332001)

manufacturer/tradename

Roche

optimum pH

4.6-4.8

storage temp.

2-8°C

General description

Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase. It is synthesized by several Aspergillus genus species. This exo-enzyme is one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.

Application

Amyloglucosidase from Aspergillus niger is used for the determination of starch.

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.
Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:
  • Western blot: 1 to 10 μg/ml


Working solution: Tris-buffered saline containing 0.1% Tween 20.

Storage conditions (working solution): 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.

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This Item
A7095A1602345386
description

from Aspergillus niger

description

≥260 U/mL, aqueous solution

description

ammonium sulfate suspension, ≥40 units/mg protein

description

Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid.

specific activity

6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate), ~6 units/mg protein (At 25 °C with glycogen as the substrate.)

specific activity

≥260 U/mL

specific activity

≥40 units/mg protein

specific activity

≥250 units/mg solid

biological source

Aspergillus niger

biological source

Aspergillus niger

biological source

-

biological source

-

form

lyophilized

form

aqueous solution

form

ammonium sulfate suspension

form

lyophilized solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

97 kDa

mol wt

-

mol wt

-

mol wt

-

optimum pH

4.6-4.8

optimum pH

-

optimum pH

-

optimum pH

-


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash



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