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Amyloglucosidase

Name: Amyloglucosidase
Brand: ROCHE
Price: 227 CAD

from Aspergillus niger

Synonym(s):

Aspergillus niger, disaccharidase-type a-glucosidase

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Size/SKU	Availability	Price
500 units	Please contact Customer Service for Availability	$227.00
3500 units	Please contact Customer Service for Availability	$640.00

About This Item

UNSPSC Code:

12352204

EC Number:

3.2.1.3 (BRENDA, IUBMB)

NACRES:

NA.54

Specific activity:

6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate), ~6 units/mg protein (At 25 °C with glycogen as the substrate.)

Biological source:

Aspergillus niger

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Properties

biological source

Aspergillus niger

form

lyophilized

specific activity

6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate), ~6 units/mg protein (At 25 °C with glycogen as the substrate.)

mol wt

97 kDa

packaging

pkg of 3,500 U (11202367001), pkg of 500 U (11202332001)

manufacturer/tradename

Roche

optimum pH

4.6-4.8

storage temp.

2-8°C

Description

General description

Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase. It is synthesized by several Aspergillus genus species. This exo-enzyme is one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.

Application

Amyloglucosidase from Aspergillus niger is used for the determination of starch.

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:

Western blot: 1 to 10 μg/ml

Working solution: Tris-buffered saline containing 0.1% Tween 20.

Storage conditions (working solution): 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.

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This Item	10115	A2910	A7095
Roche ROAMYGLL Amyloglucosidase	Sigma-Aldrich 10115 Amyloglucosidase from Aspergillus niger	Sigma-Aldrich A2910 Amyloglucosidase from Aspergillus niger	Sigma-Aldrich A7095 Amyloglucosidase from Aspergillus niger
description from Aspergillus niger	description lyophilized, powder, ~70 U/mg	description Isoelectric focusing marker, pI 3.6	description ≥260 U/mL, aqueous solution
biological source Aspergillus niger	biological source -	biological source -	biological source Aspergillus niger
specific activity 6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate), ~6 units/mg protein (At 25 °C with glycogen as the substrate.)	specific activity ~70 U/mg	specific activity -	specific activity ≥260 U/mL
form lyophilized	form powder	form powder	form aqueous solution
mol wt 97 kDa	mol wt M_r ~97,000	mol wt -	mol wt -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
optimum pH 4.6-4.8	optimum pH -	optimum pH -	optimum pH -

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Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P304 + P340 - P342 + P311 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)