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67996

Tris Acetate-EDTA buffer Concentrate

for Northern and Southern blotting, solution

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10 L
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$1,830.00

About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

$1,830.00

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Diagnostics Manufacturers should use alternative grade product: SRE0033
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grade

Molecular Biology

form

solution

quality

50x Concentrate

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

pH

8.3±0.2 (25 °C)

density

1.09 g/mL at 20 °C

storage temp.

room temp

General description

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.

Application

Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.

Preparation Note

The TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water.

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Tris Acetate-EDTA buffer Concentrate for Northern and Southern blotting, solution

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Tris Acetate-EDTA buffer pH 8.3, pHast Pack™, powder

Sigma-Aldrich

PPB008

Tris Acetate-EDTA buffer

description

for Northern and Southern blotting, solution

description

for Northern and Southern blotting, solution

description

for Northern and Southern blotting, solution

description

pH 8.3, pHast Pack™, powder

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

-

form

solution

form

solution

form

solution

form

powder

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

-

density

1.09 g/mL at 20 °C

density

-

density

-

density

-

pH

8.3±0.2 (25 °C)

pH

-

pH

8.6-9.0

pH

8.3

impurities

DNases, none detected, phosphatases, none detected, proteases, none detected, RNases, none detected

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

RNases, none detected, phosphatases, none detected, proteases, none detected, DNases, none detected

impurities

-

Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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