Yes, this product may be considered a 1X TE Buffer. This solution contains 10 mM Tris and 1 mM EDTA. Typical 10X solutions contain 100 mM Tris, 10 mM EDTA, see product PPB010 to review a 10X option.
93283
Tris-EDTA buffer solution
BioUltra, Molecular Biology, pH 8.0
Synonym(s):
TE buffer, TE buffer solution
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| Size/SKU | Availability | Price |
|---|---|---|
100 mL | Check Cart for Availability | $31.20 |
500 mL | Check Cart for Availability | $122.00 |
About This Item
UNSPSC Code:
41105319
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25
MDL number:
$31.20
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Ships Every 4 weeks
grade
Molecular Biology
Quality Segment
product line
BioUltra
form
liquid
impurities
DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
pH
8.0±0.2 (25 °C)
cation traces
Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
λ
neat
UV absorption
λ: 260 nm Amax: 0.04, λ: 280 nm Amax: 0.01
application(s)
sample preparation
SMILES string
NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI key
VLEIUWBSEKKKFX-UHFFFAOYSA-N
General description
Tris EDTA is a biological buffer in research. It is a combination of Tris base and EDTA, a metal chelator. Tris provides buffering capacity, maintaining a stable pH in biological samples, while EDTA helps prevent metal ion interference by chelating divalent metal ions. Tris EDTA is often used in molecular biology techniques, such as DNA and RNA sample preparation, electrophoresis, and enzymatic reactions. It helps maintain the stability and integrity of nucleic acids by inhibiting the activity of nucleases and metal-dependent enzymes.
Application
TE buffer is useful for general extraction, suspension, washing, and storage buffer and in the preparation of DNA or RNA. Commonly used for DNA sequencing, restriction enzyme digestion; ligation and polymerase chain reaction (PCR) applications.
Features and Benefits
- BioUltra grade - suitable for many molecular biology applications
- DNase, RNase, phosphatase, and protease-free
- High purity and free of trace metals
Other Notes
For additional information on our range of Biochemicals, please complete this form.
1 of 1
This Item | |||
|---|---|---|---|
| description BioUltra, Molecular Biology, pH 8.0 | description for Northern and Southern blotting, solution | description for Northern and Southern blotting, solution | description pH 8.3, pHast Pack™, powder |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade - |
| Quality Level 200 | Quality Level - | Quality Level 100 | Quality Level - |
| product line BioUltra | product line - | product line BioReagent | product line pHast Pack™ |
| application(s) sample preparation | application(s) - | application(s) - | application(s) - |
| form liquid | form solution | form solution | form powder |
| pH 8.0±0.2 (25 °C) | pH 8.3±0.2 (25 °C) | pH 8.6-9.0 | pH 8.3 |
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Storage Class
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves
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Is this product 1x ?
1 answer-
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Is this product sterile and pyrogen free?
1 answer-
This TE buffer solutions is not tested for pyrogen content, and it is not tested for sterility. Since it is not autoclaved or sterile filtered, it would be best to assume that it's not sterile as provided. This solution is recommended for Molecular Biology applications, which may not require the use of a pyrogen-free and/or sterile solution.
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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Is this buffer composed by 10mM Tris and 1 mM EDTA?
1 answer-
This buffer is comprised of 10 mM Tris-HCl and 1 mM disodium EDTA, pH 8.0.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the storage condition for TE buffer BioUltra
1 answer-
This product may be stored at room temperature.
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What is the concentration of Tris and EDTA in this buffer?
1 answer-
This buffer is 10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0.
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