A3643
Agarose
Type IV, Special High EEO
Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE
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About This Item
biological source
algae (marine)
Quality Segment
type
Type IV
form
powder
technique(s)
electrophoresis: suitable
EEO
≥0.3
SMILES string
O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
Application
Analysis Note
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
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This Item | |||
|---|---|---|---|
| description Type IV, Special High EEO | description Ultra-low Gelling Temperature | description Type I-A, low EEO | description Wide range, Molecular Biology |
| EEO ≥0.3 | EEO ≤0.05 | EEO 0.09-0.13 | EEO ≤0.15 |
| form powder | form powder | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) electrophoresis: suitable |
| biological source algae (marine) | biological source algae (marine) | biological source - | biological source algae (marine) |
| type Type IV | type Type IX | type Type I-A | type - |
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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