If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
A4018
Agarose, low gelling temperature
Synonym(s):
2-Hydroxyethyl agarose
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| Size/SKU | Availability | Price |
|---|
About This Item
biological source
algae (marine)
Quality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
EEO
≤0.10
transition temp
congealing temperature 26-30 °C
gel strength
≥200 g/cm2 (1% gel)
SMILES string
O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
General description
Application
Agarose, low gelling temperature has been used:
- to immobilize zebra fish embryos during in vivo imaging experiments
- as a constituent of defined medium to culture bovine nucleus pulposus (NP) cells
- as a hydrogel, a scaffolding material for dental pulp regeneration
- in the form of pellets to determine the surface pH and conductivity of an acrylic emulsion film
Analysis Note
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
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This Item | |||
|---|---|---|---|
| description - | description BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture | description BioReagent, Molecular Biology | description Type VII-A |
| gel strength ≥200 g/cm2 (1% gel) | gel strength ≥200 g/cm2 (1% gel) | gel strength ≥200 g/cm2 (1% gel) | gel strength ≥250 g/cm2 (1% gel) |
| form powder | form powder | form powder | form powder |
| EEO ≤0.10 | EEO ≤0.1 | EEO ≤0.10 | EEO ≤0.12 |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | insect: suitable, cell culture | plant: suitable, cell culture | mammalian: suitable | technique(s) - | technique(s) electrophoresis: suitable |
| biological source algae (marine) | biological source - | biological source algae (red) | biological source - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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In order to have sterile Low Melting Agarose, once the package has already been opened outside of sterile conditionds, should I filter it and then lyophilize or should I use an autoclave?
1 answer-
Agarose solutions can be autoclaved.
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