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PPB002

Tris Buffered Saline

powder, pH 8.0, pHast Pack™, with 0.05% Tween® 20

Synonym(s):

Tris Buffered Saline, with Tween™ 20, pH 8.0, TBS with 0.05% Tween-20, TBS-T, TBST

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Size/SKUAvailabilityPrice
20 pkg
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$161.00

About This Item

UNSPSC Code:
41105323
NACRES:
NA.21

$161.00

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Product Name

Tris Buffered Saline, with 0.05% Tween® 20, pH 8.0, powder, pHast Pack™

product line

pHast Pack™

form

powder

storage condition

(Tightly closed. Dry.)

impurities

≤5 ppm Heavy Metals (lead)

color

white

pH

7.8—8.2

solubility

soluble, clear to hazy, colorless

cation traces

Fe: ≤10 ppm

foreign activity

DNase, none detected, Endonuclease , Exonuclease, none detected, NICKase, none detected, Protease, none detected, RNase, none detected

storage temp.

room temp

General description

Tris Buffered Saline with 0.05% Tween® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.

Application

Suitable for use as a wash buffer in:
  • Western blot
  • ELISA
  • Immunohistochemistry

Features and Benefits

  • Ready-to-use buffer requires no measurments or pH adjustments
  • Saves time, money and effort to get your assays started fast
  • Physical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and Nickase
  • Qualified for use in gel electrophoreis and other molecular biology assays

Packaging

Foil pouches

Preparation Note

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC
pHast Pack is a trademark of Sigma-Aldrich Co. LLC

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Tris Buffered Saline, with Tween™ 20, pH 8.0

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Tris Buffered Saline pH 7.6, pHast Pack™, powder

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PPB011

Tris Buffered Saline

description

powder, pHast Pack™

description

powder

description

pH 8.0, pHast Pack™, powder

description

pH 7.6, pHast Pack™, powder

product line

pHast Pack™

product line

-

product line

pHast Pack™

product line

pHast Pack™

form

powder

form

powder

form

powder

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

room temp

solubility

soluble, clear to hazy, colorless

solubility

-

solubility

soluble, clear, colorless

solubility

soluble, clear, colorless

storage condition

(Tightly closed. Dry.)

storage condition

-

storage condition

(Tightly closed. Dry)

storage condition

(Tightly closed. Dry)

pH

7.8—8.2

pH

-

pH

8.0

pH

7.6

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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