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S9305

SYBR™ Green II RNA gel stain

10,000 × in DMSO

Sustainability features

Synonym(s):

RNA gel dye, safer gel stain, SYBR™ RNA dye

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Size/SKUAvailabilityPrice
0.5 mL
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$787.00

About This Item

CAS Number:
UNSPSC Code:
12171500
EC Number:
200-664-3
NACRES:
NA.52
MDL number:

$787.00


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Quality Segment

usage

 mL sufficient for 100 mini-gels

sustainability

Sustainability features

concentration

10,000 × in DMSO

greener alternative category

storage temp.

−20°C

General description

SYBR™ Green II is a highly sensitive stain for post electrophoresis staining of RNA and ssDNA in agarose or polyacrylamide gels. SYBR™ Green II is not selective for RNA staining but does exhibit a higher quantum yield when bound to RNA than to double stranded DNA.
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has inherently safer chemistry, compared to the standard use of ethidium bromide for staining. For more information see publication found in related content.

Application

SYBR™ Green II RNA gel stain has been used:

  • for the quantification of ribonucleic acid (RNA) in Escherichia coli
  • for staining 5′-ETS rRNA species separated on polyacrylamide gel prior to Northern blot analysis
  • to stain formaldehyde agarose gels to visualize RNA bands

Features and Benefits

  • Ultrasensitive stain for post-electrophoresis staining of RNA and ssDNA
  • Excited maximally at 497 nm with a secondary excitation peak at 254nm
  • The fluorescence emission of SYBR™Green II stained RNA is centered at 520 nm
  • Staining agarose/formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or subsequent hybridization in Northern blot analysis as long as 0.1% -0.3% SDS is included in prehybridization and hybridization buffers to remove the dye
  • Might facilitate the detection of viroid RNAs and multicopy cellular RNA species
  • More sensitive than ethidium bromide in applications that require extremely sensitive detection techniques such as single-strand conformation polymorphism (SSCP) analysis

Legal Information

SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries

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S9430XNATRGT3073
description

10,000 × in DMSO

description

10,000 × in DMSO

description

sufficient for 1000 extractions, sufficient for 1000 amplifications

description

30 mL sufficient for 1,000 extractions

concentration

10,000 × in DMSO

concentration

10,000 × in DMSO

concentration

-

concentration

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

greener alternative category

Aligned,

greener alternative category

Aligned,

greener alternative category

-

greener alternative category

-

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

greener alternative product characteristics

-

usage

 mL sufficient for 100 mini-gels

usage

 mL sufficient for 100 mini-gels

usage

sufficient for 1000 amplifications, sufficient for 1000 extractions, sufficient for 1000 reactions

usage

sufficient for 1000 reactions, 30 mL sufficient for 1,000 extractions


PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Storage Class

10 - Combustible liquids

Flash Point (°F)

188.6 °F - closed cup

Flash Point (°C)

87 °C - closed cup



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