04963
Lyticase from Bacillus subtilis
≥500 U/mL
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 g | Check Cart for Availability | $59.90 | |
| 5 g | Check Cart for Availability | $227.00 |
About This Item
Specific activity:
≥500 U/mL
$59.90
Check Cart for Availability
Quality Segment
form
liquid, viscous liquid
specific activity
≥500 U/mL
solubility
H2O: 1:10, clear, colorless to very faintly green
suitability
corresponds for SDS-PAGE
storage temp.
−20°C
General description
Lyticase from Bacillus subtilis is a hydrolytic endoglycosidase that specifically cleaves β(1-3)-glucan linkages found in the cell walls and biofilm matrices of fungi and certain bacteria. Lyticase enzyme acts by degrading the structural glucan scaffold of microbial cell walls, enabling the generation of spheroplasts and facilitating nucleic acid extraction from yeast cells.
Application
Lyticase from Bacillus subtilis has been used for:
- digesting yeast cell walls to convert intact cells into spheroplasts enabling subsequent membrane isolation.
- enhancing antimicrobial treatment against interkingdom biofilms when combined with an enzymatic cocktail (cellulase, denarase, and dispersin B) prior to antimicrobial application.
Biochem/physiol Actions
Lyticase cleaves β(1-3) bonds within the glucose units of fungal cell walls and bacterial biofilm matrices, including the Psl polysaccharide of Pseudomonas aeruginosa breaking them down into smaller sugar fragments and dismantling the structural backbone of the cell. It dissolves the yeast (S. cerevisiae) cell wall, measurable as a decrease in optical turbidity at 800 nm (pH 7.5, 25°C). Lyticase requires two components to fully lyse cells: a glucanase that destroys the glucan scaffold, and an alkaline protease that removes the outer mannoprotein layer blocking glucanase access. By breaking down the C. albicans cell wall, lyticase increases permeability and enhances the killing effect of antifungal drugs such as caspofungin.
Other Notes
1U corresponds to the amount of enzyme which decreases the absorbance at 800 nm by 0.001 per minute at pH 7.5 and 25 C (Yeast, Cat. No. 51475, as substrate).
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥500 U/mL | specific activity ≥2000 units/mg protein | specific activity ≥200 units/mg solid | specific activity ≥2000 units/mg protein |
| form liquid, viscous liquid | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| suitability corresponds for SDS-PAGE | suitability - | suitability - | suitability - |
| solubility H2O: 1:10, clear, colorless to very faintly green | solubility - | solubility - | solubility - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 300 | Quality Level 200 | Quality Level 400 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 151483-25G | 04061838739247 |
| 151483-100G | 04061833554326 |
| 04963-5G | 04061838635938 |
| 04963-25G | 04061838635921 |
| 04963-1G | 04061838635914 |