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04963

Lyticase from Bacillus subtilis

≥500 U/mL

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1 g
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$59.90
5 g
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$227.00

About This Item

CAS Number:
144941-36-6
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥500 U/mL

$59.90

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Quality Segment

100

form

liquid, viscous liquid

specific activity

≥500 U/mL

input

plant, fungi and insect sample(s)

solubility

H2O: 1:10, clear, colorless to very faintly green

suitability

corresponds for SDS-PAGE

storage temp.

−20°C

General description

Lyticase from Bacillus subtilis is a hydrolytic endoglycosidase that specifically cleaves β(1-3)-glucan linkages found in the cell walls and biofilm matrices of fungi and certain bacteria. Lyticase enzyme acts by degrading the structural glucan scaffold of microbial cell walls, enabling the generation of spheroplasts and facilitating nucleic acid extraction from yeast cells.

Application

Lyticase from Bacillus subtilis has been used for:
  • digesting yeast cell walls to convert intact cells into spheroplasts enabling subsequent membrane isolation.
  • enhancing antimicrobial treatment against interkingdom biofilms when combined with an enzymatic cocktail (cellulase, denarase, and dispersin B) prior to antimicrobial application.

Biochem/physiol Actions

Lyticase cleaves β(1-3) bonds within the glucose units of fungal cell walls and bacterial biofilm matrices, including the Psl polysaccharide of Pseudomonas aeruginosa breaking them down into smaller sugar fragments and dismantling the structural backbone of the cell. It dissolves the yeast (S. cerevisiae) cell wall, measurable as a decrease in optical turbidity at 800 nm (pH 7.5, 25°C). Lyticase requires two components to fully lyse cells: a glucanase that destroys the glucan scaffold, and an alkaline protease that removes the outer mannoprotein layer blocking glucanase access. By breaking down the C. albicans cell wall, lyticase increases permeability and enhances the killing effect of antifungal drugs such as caspofungin.

Other Notes

1U corresponds to the amount of enzyme which decreases the absorbance at 800 nm by 0.001 per minute at pH 7.5 and 25 C (Yeast, Cat. No. 51475, as substrate).

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Lyticase from Bacillus subtilis ≥500 U/mL

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Lyticase from Arthrobacter Luteus Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein

Sigma-Aldrich

SRE0018

Lyticase from Arthrobacter Luteus

description

≥500 U/mL

description

free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder

description

lyophilized powder, ≥200 units/mg solid

description

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥2000 units/mg protein

specific activity

≥500 U/mL

specific activity

≥2000 units/mg protein

specific activity

≥200 units/mg solid

specific activity

≥2000 units/mg protein

form

liquid, viscous liquid

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

suitability

corresponds for SDS-PAGE

suitability

-

suitability

-

suitability

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

H2O: 1:10, clear, colorless to very faintly green

solubility

-

solubility

-

solubility

-

Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

400

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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