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05066

Agarose

High EEO

Synonym(s):

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose HE

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Pack SizeSKUAvailabilityPrice
50 g

Available to ship TODAYfromMILWAUKEE

$237.00
500 g

Available to ship TODAYfromMILWAUKEE

$1,500.00

About This Item

CAS Number:
UNSPSC Code:
41105317
PubChem Substance ID:
EC Number:
232-731-8
NACRES:
NA.25
MDL number:

$237.00


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grade

Molecular Biology

Quality Level

form

powder

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

ign. residue

≤1%

loss

≤10% loss on drying

EEO

0.23-0.27

transition temp

gel point 34-37 °C (1.5% solution)

gel strength

≥1500 g/cm2

anion traces

chloride (Cl-): ≤0.3%, sulfate (SO42-): ≤0.6%

cation traces

Ca: ≤0.05%, Cd: ≤0.001%, Co: ≤0.001%, Cr: ≤0.001%, Cu: ≤0.001%, Fe: ≤0.001%, K: ≤0.05%, Mg: ≤0.001%, Mn: ≤0.001%, Na: ≤0.5%, Ni: ≤0.001%, Pb: ≤0.001%, Zn: ≤0.001%

SMILES string

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

Application

Agarose is used for preparative and analytical separation of nucleic acids. It can be used for single cell gel electrophoresis assay.[1] It can also be used for southern blotting. It can also be used for assessing transepithelial ionic fluxes from cultured neonatal rat semicircular canal epithelium.[2]

Biochem/physiol Actions

Agarose is a polysaccharide used for resolving DNA and RNA fragments from 500 - 20,000 bp. It provides strong gel structure which assists in better handling and less breakage.[3] It can also reexpress the differentiated collagen phenotype from dedifferentiated chondrocytes during agarose gel culture.[4]

Packaging

Packaging
50, 500 g in poly bottle

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

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This Item
A5304A3643A6013
form

powder

form

powder

form

powder

form

powder

grade

Molecular Biology

grade

-

grade

-

grade

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

impurities

DNases, none detected, phosphatases, none detected, RNases, none detected, proteases, none detected

impurities

≤11% moisture content

impurities

-

impurities

≤10% water

transition temp

gel point 34-37 °C (1.5% solution)

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

transition temp

-

transition temp

gel point 36 °C ±1.5 °C (1.5% gel)

loss

≤10% loss on drying

loss

-

loss

-

loss

-


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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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C M Hughes et al.
Molecular human reproduction, 2(8), 613-619 (1996-08-01)
Baseline DNA damage in spermatozoa from fertile and infertile men was compared using a modified alkali single cell gel electrophoresis (comet) assay. Semen from normozoospermic fertile, normozoospermic infertile and asthenozoospermic infertile (World Health Organization criteria, 1992) samples were studied. No
P D Benya et al.
Cell, 30(1), 215-224 (1982-08-01)
The differentiated phenotype of rabbit articular chondrocytes consists primarily of type II collagen and cartilage-specific proteoglycan. During serial monolayer culture this phenotype is lost and replaced by a complex collagen phenotype consisting predominately of type I collagen and a low
Pierre G Milhaud et al.
American journal of physiology. Cell physiology, 283(6), C1752-C1760 (2002-10-22)
The ductal epithelium of the semicircular canal forms much of the boundary between the K+-rich luminal fluid and the Na+-rich abluminal fluid. We sought to determine whether the net ion flux producing the apical-to-basal short-circuit current (I(sc)) in primary cultures



Global Trade Item Number

SKUGTIN
T130500004061833801130
T34002-25G04061837344398
05066-50G04061835540938
05066-500G04061838636546

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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