10065
α-Amylase from Aspergillus oryzae
powder, ~30 U/mg
Synonym(s):
1,4-α-D-Glucan-glucanohydrolase
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| Size/SKU | Availability | Price |
|---|---|---|
10 g | Check Cart for Availability | $87.30 |
50 g | Please contact Customer Service for Availability | $359.00 |
About This Item
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-588-1
NACRES:
NA.54
MDL number:
Specific activity:
~30 U/mg
General description
Microbial amylases are exoenzymes and are used in several industrial applications, such as production of bread, maltose syrups, and fermentation of soya sauce, miso etc. α-Amylase isolated from Aspergillus oryzae has been found to have molecular weights of 51kDa (sedimentation and diffusion) and 49kDa (gel filtration).
Application
Biochem/physiol Actions
Aspergillus oryzae α-amylase (Ao α-amylase) enzyme catalyzes the hydrolysis of the α-1,4 glycosidic bonds in soluble starches and related subsrates. These substrates are broken down to release short oligosaccharides and α-limit dextrins.[4]
Other Notes
1 U corresponds to the amount of enzyme which liberates 1 μmol maltose per minute at pH 6.0 and 25°C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).
For the determination of fats in food; Application in (selective) hydrolysis/condensation of glycosidic bonds.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ~30 U/mg | specific activity ~1.5 U/mg (~0.2 U acc. to Willstätter) | specific activity ≥150 units/mg protein (biuret) | specific activity ≥800 FAU/g |
| form powder | form powder | form powder | form aqueous solution |
| mol wt Mr ~51000 | mol wt - | mol wt - | mol wt - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
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Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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