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21879

5(6)-Carboxyfluorescein diacetate

BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

Synonym(s):

5(6)-Carboxy-di-O-acetylfluorescein

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Size/SKUAvailabilityPrice
25 mg
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$91.00
100 mg
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$304.00

About This Item

Empirical Formula (Hill Notation):
C25H16O9
CAS Number:
Molecular Weight:
460.39
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352108
MDL number:

$91.00


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product line

BioReagent

Quality Segment

assay

≥90.0% (HPLC)

form

powder

solubility

DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1.CC(=O)Oc6ccc7c(Oc8cc(OC(C)=O)ccc8C79OC(=O)c%10cccc(C(O)=O)c9%10)c6

InChI

1S/2C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25;1-12(26)31-14-6-8-18-20(10-14)33-21-11-15(32-13(2)27)7-9-19(21)25(18)22-16(23(28)29)4-3-5-17(22)24(30)34-25/h2*3-11H,1-2H3,(H,28,29)

InChI key

SWRGCNMDGRMQGB-UHFFFAOYSA-N

General description

5(6)-Carboxyfluorescein diacetate or CDFDA is a fluorescent oxidant sensing probe. It is an intracellular probe added to the cells as a pro-substrate, which upon hydrolysis by the esterase enzyme produces the fluorescent product called CDF.

Application

5(6)-Carboxyfluorescein diacetate (CFDA) is a dye which is been extensively utilized to label cultured neurons with the essential requirements, in a specific manner.

5(6)- Carboxyfluorescein diacetate (CFDA) is a viability probe that assesses enzymatic activity, which is necessary to activate its fluorescence, and cell-membrane integrity, which is necessary for the retention of their fluorescent product within the cell.

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This Item
C5041F7378
description

BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

description

≥95% (HPLC)

description

used as cell viability stain

suitability

suitable for fluorescence

suitability

-

suitability

-

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

-

fluorescence

-

solubility

DMSO: soluble

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow

solubility

acetone: 25 mg/mL

form

powder

form

powder

form

powder

Quality Level

100

Quality Level

200, 100

Quality Level

200

assay

≥90.0% (HPLC)

assay

≥95% (HPLC)

assay

-


Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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