Giemsa solution should never be diluted in PBS. Giemsa solution is typically diluted with either deionized water or a low molarity phosphate buffer. Weise and Sorsensen phosphate buffer solutions are typically used. Dilutions can range from 1:10 to 1:80. The most common range for dilutions are either 1:20 or 1:40.
32884
Giemsa Stain
Modified Solution
Synonym(s):
Azure eosin methylene blue, Giemsa solution
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| Size/SKU | Availability | Price |
|---|---|---|
250 mL | Check Cart for Availability | $46.20 |
1 L | Check Cart for Availability | $130.00 |
About This Item
CAS Number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
MA.02
MDL number:
$46.20
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Product Name
Giemsa Stain, Modified Solution, according to Giemsa
form
liquid
Quality Segment
quality
according to Giemsa
technique(s)
UV/Vis spectroscopy: suitable
color
dark blue
pH
6.9
density
1.036 g/cm3 at 20 °C
suitability
passes test for UV spectrum
application(s)
diagnostic assay manufacturing
hematology
histology
microbiology
storage temp.
room temp
SMILES string
[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
InChI key
NALREUIWICQLPS-UHFFFAOYSA-N
General description
Application
Biochem/physiol Actions
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
Other Notes
Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.
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This Item | |||
|---|---|---|---|
| description according to Giemsa | description (for the staining (of cellular blood components and blood parasites)) | description technical grade, used as a blood stain | description - |
| suitability passes test for UV spectrum | suitability spirochetes, Histoplasma spp., Plasmodium spp., protozoa, bacteria, Trichomonas spp., Chlamydia spp. | suitability application for blood stain | suitability - |
| technique(s) UV/Vis spectroscopy: suitable | technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - |
| color dark blue | color - | color dark greenvery dark | color - |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) hematology |
| form liquid | form - | form powder | form solution |
| storage temp. room temp | storage temp. - | storage temp. room temp | storage temp. room temp |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
Target Organs
Eyes,Central nervous system
Storage Class
3 - Flammable liquids
WGK
WGK 2
Flash Point (°F)
51.8 °F - closed cup
Flash Point (°C)
11 °C - closed cup
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To stain a blood smear does this product need to be diluted in PBS? What is the recommended ratio of stain to PBS?
1 answer-
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Hello I would like to ask if this product is suitable for G banding of human chromosomes. Thank you very much!
1 answer-
Product 32884 has not been suitability tested for G banding of human chromosomes. There is no available protocol for use of the product for G banding of human chromosomes. The quality control testing performed on a lot-to-lot basis includes testing for appearance, density, pH, and UV absorbance.
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