The material should be soluble in ethanol, with dichloromethane (DCM) being a commonly used solvent for it.
37291
Dihydroethidium
BioReagent, suitable for fluorescence, ≥95% (HPCE)
Synonym(s):
2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 mg | Check Cart for Availability | $390.00 | |
| 100 mg | Please contact Customer Service for Availability | $993.00 |
About This Item
Empirical Formula (Hill Notation):
C21H21N3
CAS Number:
Molecular Weight:
315.41
UNSPSC Code:
12161505
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
5107482
product line
BioReagent
Quality Level
assay
≥95% (HPCE)
form
solid
mol wt
315.41 g/mol
solubility
acetonitrile: soluble, methanol: soluble
fluorescence
λex 358 nm; λem 461 nm, λex 392 nm; λem 410 nm in methanol
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14
InChI
1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
InChI key
XYJODUBPWNZLML-UHFFFAOYSA-N
Application
Dihydroethidium (dHEt), a fluorescene probe, is used to detect the presence of reactive oxygen species (ROS) such as superoxides.
Redox indicator. Blue fluorescence until oxidized to ethidium.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
B-ring reduced analog of ethidium
1 of 1
This Item | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability suitable for fluorescence | suitability - |
| fluorescence λex 358 nm; λem 461 nm, λex 392 nm; λem 410 nm in methanol | fluorescence λex 370 nm; λem 420 nm (for cytoplasm of living cells), λex 535 nm; λem 610 nm (for chromatin of living cells) | fluorescence λex 367 nm; λem 445 nm in 0.1 M phosphate pH 7.0 (after derivatization with pyruvate) | fluorescence λex 360 nm; λem 590 nm in PBS, λex 526 nm; λem 605 nm (10 mM TBE; pH 8.0;), λex 526 nm; λem 605 nm in aqueous buffer |
| solubility acetonitrile: soluble, methanol: soluble | solubility chloroform: 20 mg/mL | solubility - | solubility - |
| form solid | form powder | form solid | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
| assay ≥95% (HPCE) | assay ≥95% | assay ≥97.0% (HPLC) | assay - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Rossarin Suwanarusk et al.
The Journal of antimicrobial chemotherapy, 70(1), 124-129 (2014-08-26)
Methylene blue, once discarded due to its unsettling yet mild side effects, has now found a renewed place in the pharmacopoeia of modern medicine. The continued spread of drug-resistant Plasmodium vivax and Plasmodium falciparum has also led to a recent
Karina A Roth et al.
Journal of endodontics, 38(9), 1253-1256 (2012-08-16)
Root canal fillings are intended to prevent microbial proliferation over time in the canal after treatment. The objective of this study was to assess biofilm proliferation within the sealer-dentin interfaces of 2 methacrylate resin-based systems, self-etch (SE) and total-etch (TE)
Francisco R M Laurindo et al.
Methods in enzymology, 441, 237-260 (2008-06-17)
Assessment of low-level superoxide in nonphagocytic cells is crucial for assessing redox-dependent signaling pathways and the role of enzymes such as the NADPH oxidase complex. However, most superoxide probes present inherent limitations. Particularly, assessment of dihydroethidium (DHE) fluorescence is limited
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 15382-25G | 04061838741141 |
| 15382-5G | 04061832593715 |
| 8530570100 | 04027269098254 |
| 15382-100G | 04061832593708 |
| 8530570025 | 04027269098247 |
| 37291-100MG | 04061833488911 |
| 37291-25MG | 04061831834352 |
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