Yes, this product is stable until recommended retest date in original container. This product is a prepared solution of 1M DTT in water. In the original sealed container it is quite stable, with an assigned retest schedule of every 2 years. Assuming that the product is handled properly, the material is expected to be stable until at least the retest date reported on the lot specific Certificate of Analysis.
43816
DL-Dithiothreitol solution
BioUltra, Molecular Biology, ~1 M in H2O
Sinonimo/i:
Cleland′s reagent, (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, (±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
10 mL | Per conoscere la disponibilità, visualizza il carrello | CHF 43.40 |
50 mL | Per conoscere la disponibilità, visualizza il carrello | CHF 148.00 |
250 mL | Per conoscere la disponibilità, visualizza il carrello | CHF 468.00 |
Informazioni su questo articolo
Formula empirica (notazione di Hill):
C4H10O2S2
Numero CAS:
Peso molecolare:
154.25
UNSPSC Code:
12352201
NACRES:
NA.25
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1719757
Servizio Tecnico
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Molecular Biology
Quality Segment
product line
BioUltra
assay
900-1100 mmol/L (Titration molarity)
form
liquid
reaction suitability
reagent type: reductant
concentration
~1 M in H2O
impurities
DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
pH
2.7-5.0
density
1.04 g/mL at 20 °C
absorption
cut-off at 330 nm
suitability
in accordance for filter test, suitable for molecular biology
storage temp.
2-8°C
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
General description
Dithiothreitol (DTT), a thiol group protectant,[1] is an excellent reagent for preventing the oxidation of SH groups in proteins. DTT solution is a potent and versatile disulfide-reducing agent.[2] It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange.
Application
DL-dithiothreitol solution has been used:
- in the treatment of intestinal explants
- in homogenate preparation of gill cells for enzymatic assays
- in protein electrophoresis for western blotting
- in transglutaminase activity
- in sample buffer for SDS-PAGE
- in Western Blot, as a lysis buffer component for single-nucleus RNA-sequencing
- as a component of protein extraction buffer for cell lysis
Biochem/physiol Actions
DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms. It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.Dithiothreitol (DTT) is broadly utilized in synthetic peptide synthesis and biochemical arrangements of thiol proteins. It can also be used to study protein chemistry topics like enzyme activity and protein folding. DTT explicitly controls the thiol-disulfide exchange response to completely reduce the intra-or between sub-atomic disulfide bonds in biomolecules. Thiols and the cyclic disulfide of DTT are the products of this reaction.
Features and Benefits
- BioUltra grade - 1M DTT solution, suitable for molecular biology
- Free from DNases, RNases, phosphatases, and proteases
Other Notes
For additional information on our range of Biochemicals, please complete this form.
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Questo articolo | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade - | grade - |
| assay 900-1100 mmol/L (Titration molarity) | assay ≥99.5% (RT) | assay ≥98% (HPLC), ≥99.0% (titration) | assay ≥99% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| product line BioUltra | product line BioUltra | product line - | product line - |
| pH 2.7-5.0 | pH 4.0-6.5 (25 °C, 0.1 M in H2O) | pH - | pH - |
| impurities DNases, none detected, phosphatases, none detected, RNases, none detected, proteases, none detected | impurities DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.3% 4,5-dihydroxy-1,2-dithiane | impurities - | impurities - |
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Danger
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Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Classe di stoccaggio
8A - Combustible corrosive hazardous materials
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After opening DTT ref 43816-50ML, is the product stable? Can it be reused?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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How stable is this DTT solution ? it is known that DTT is unstable in solution and should be kept under inert atmosphere. How long can I keep it once open ?
1 answer-
This product is a prepared solution of 1M DTT in water. In the original sealed container it is quite stable, with an assigned retest schedule of every 2 years. Assuming that the product is handled properly, the material is expected to be stable until at least the retest date reported on the lot specific Certificate of Analysis.
Helpful?
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