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46393

Fluo-3

suitable for fluorescence, ~70%

Synonym(s):

4-(2,7-Dichloro-6-hydroxy-3-oxo-9-xanthenyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid

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Pack SizeSKUAvailabilityPrice
1 mg
Please contact Customer Service for Availability
$662.00
$463.40

About This Item

Empirical Formula (Hill Notation):
C36H30Cl2N2O13
CAS Number:
123632-39-3
Molecular Weight:
769.54
NACRES:
NA.32
PubChem Substance ID:
329757162
UNSPSC Code:
12352106
MDL number:
MFCD00083337

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assay

~70%

Quality Level

100

form

solid

solubility

H2O: soluble

fluorescence

λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

Cc1ccc(N(CC(O)=O)CC(O)=O)c(OCCOc2cc(ccc2N(CC(O)=O)CC(O)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(O)c(Cl)cc35)c1

InChI

1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50)

InChI key

OZLGRUXZXMRXGP-UHFFFAOYSA-N

Application

Fluo-3 and related molecule Fluo3/AM are used as a fluorescence indicator of intracellular calcium (Ca2+). Fluo-3 may be use for flow cytometry and confocal laser scanning microscopy using visible light excitation (compatible with argon laser sources operating at 488 nm). Fluorescence intensity increases about 40-fold after calcium binding.

Other Notes

Fluorescent indicator for calcium used in flow cytometry. Fluorescence intensity increases about 40-fold after calcium binding[1][2]

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This Item
73881927082A2846
Fluo-3 suitable for fluorescence, ~70%

Sigma-Aldrich

46393

Fluo-3

Fluo 3-AM ≥90% (TLC)

Sigma-Aldrich

73881

Fluo 3-AM

Ni(COD)(Cy-BQ) ≥95%

Sigma-Aldrich

927082

Ni(COD)(Cy-BQ)

A-134974 dihydrochloride hydrate ≥98% (HPLC), solid

Sigma-Aldrich

A2846

A-134974 dihydrochloride hydrate

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

-

fluorescence

λex 506 nm; λem 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca2+

fluorescence

λex 506 nm; λem 525 nm in 0.1 M Tris pH 8.0, esterase

fluorescence

-

fluorescence

-

solubility

H2O: soluble

solubility

-

solubility

-

solubility

H2O: soluble

form

solid

form

powder

form

powder

form

solid

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

assay

~70%

assay

≥90% (TLC)

assay

≥95%

assay

≥98% (HPLC)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK5769
BCCF4708
BCCC6119
BCBW6132
BCBS0366V

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P A Vandenberghe et al.
Journal of immunological methods, 127(2), 197-205 (1990-03-09)
Cytoplasmic free calcium [( Ca2+]i) is a key intracellular messenger in many cell types. We have used fluo-3, a recently developed calcium probe, to study [Ca2+]i in resting and stimulated human peripheral blood T lymphocytes. The spectral properties of fluo-3
Xinjing Guo et al.
Food & function, 10(8), 4661-4673 (2019-07-12)
Hydroxysafflor yellow A (HSYA) is the main active ingredient of edible plant safflower. HSYA has demonstrated anti-inflammatory effects. The inflammatory response is the key mechanism responsible for asthma, and the pro-inflammatory platelet-activating factor (PAF) is known to play a role
Haili Wu et al.
Journal of agricultural and food chemistry, 64(49), 9356-9367 (2016-12-15)
Resveratrol (Res), a natural phytoalexin found in a variety of plants, has significant antitumor activity. Pyruvate kinase M2 (PKM2) has abnormally high expression in various tumor cells, and it has been implicated in the survival of tumors. However, whether and
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Global Trade Item Number

SKUGTIN
46393-5MG-F04061833277256
46393-1MG-F04061826678848

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