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Guanidine thiocyanate

Name: Guanidine thiocyanate
Brand: SIGMA
Price: 220 USD
Availability: InStock

BioReagent, Molecular Biology, ≥99%

Synonym(s):

Guanidinium rhodanide, Guanidinium thiocyanate

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Size/SKU	Availability	Price
100 g	Available to ship TODAY	$220.00
500 g	Available to ship TODAY	$786.00

About This Item

Linear Formula:

NH₂C(=NH)NH₂ · HSCN

CAS Number:

593-84-0

Molecular Weight:

118.16

UNSPSC Code:

12352107

NACRES:

NA.52

PubChem Substance ID:

57651291

EC Number:

209-812-1

Beilstein/REAXYS Number:

3563461

MDL number:

MFCD00013027

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Properties

biological source

synthetic

Quality Segment

200

grade

Molecular Biology

product line

BioReagent

assay

≥99%

form

powder

technique(s)

RNA extraction: suitable

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

color

colorless to white,

mp

115-122 °C, 120-122 °C (lit.)

solubility

H₂O: 4 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤5000 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤5000 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

storage temp.

room temp

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Description

Application

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.

Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.

Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures.

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This Item	G6639	G4505	50980
Sigma-Aldrich 50981 Guanidine thiocyanate	Sigma-Aldrich G6639 Guanidine thiocyanate	Sigma-Aldrich G4505 Guanidine hydrochloride	Sigma-Aldrich 50980 Guanidine thiocyanate
description BioReagent, Molecular Biology, ≥99%	description ≥97% (titration)	description ≥99% (titration), organic base and chaeotropic agent	description BioUltra, Molecular Biology, ≥99.0% (AT)
grade Molecular Biology	grade -	grade -	grade Molecular Biology
technique(s) RNA extraction: suitable	technique(s) RNA extraction: suitable	technique(s) RNA extraction: suitable	technique(s) -
product line BioReagent	product line -	product line -	product line BioUltra
Quality Level 200	Quality Level 200	Quality Level 400	Quality Level 100
form powder	form powder or crystals	form crystalline powder	form solid
assay ≥99%	assay ≥97% (titration)	assay ≥99% (titration)	assay ≥99.0% (AT)

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Pictograms

GHS05,GHS07

Signal Word

Danger

Hazard Codes

H302 + H312 + H332,H314,H412

Precautionary Statements

P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

supp_hazards

EUH032

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Gloves

Storage Class

8A - Combustible corrosive hazardous materials

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