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53608

Biotin (5-fluorescein) conjugate

BioReagent, suitable for fluorescence, ≥90% (HPCE)

Sinónimos:

N-(Biotinylamidoethyl)-fluorescein-5-carboxamide, N-(Fluorescein-5-carbamidoethyl)biotinamide

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A ustedes/SKUDisponibilidadPrecio
1 mg
Comprobar disponibilidad del carrito
US$ 346,00
5 mg
Comprobar disponibilidad del carrito
US$ 1.180,00
US$ 826,00

Acerca de este artículo

Fórmula empírica (notación de Hill):
C33H32N4O8S
Número CAS:
Peso molecular:
644.69
UNSPSC Code:
12352108
eCl@ss:
34058011
PubChem Substance ID:
NACRES:
NA.32
MDL number:

US$ 346,00

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product line

BioReagent

Quality Segment

assay

≥90% (HPCE)

solubility

DMSO: soluble

fluorescence

λex 490 nm; λem 524 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NCCNC(=O)c3ccc(c(c3)C(O)=O)C4=C5C=CC(=O)C=C5Oc6cc(O)ccc46)[C@@]1([H])NC(=O)N2

InChI

1S/C33H32N4O8S/c38-18-6-9-21-25(14-18)45-26-15-19(39)7-10-22(26)29(21)20-8-5-17(13-23(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30-/m0/s1

InChI key

DFUFXKZUEOKPSD-LFERIPGTSA-N

Application

Biotin (5-fluorescein) conjugate is a reagent that may be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Biotin-fluorescein conjugate for the fluorometric, accurate measurement of avidin and streptavidin.
Often referred to as Biotin (4-fluorescein) conjugate.

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Este artículo
469394693588596
suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence

suitability

suitable for fluorescence

fluorescence

λex 490 nm; λem 524 nm in 0.1 M Tris pH 8.0

fluorescence

λex 492 nm; λem 520 nm in 0.1 M phosphate pH 7.0 (after derivatization with 2-mercaptoethanol and after cleavage by esterase)

fluorescence

λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

fluorescence

λex 455 nm; λem 514 nm in 0.1 M Tris pH 8.0

solubility

DMSO: soluble

solubility

DMF: soluble, DMSO: soluble, dioxane: soluble

solubility

DMF: soluble, DMSO: soluble

solubility

-

assay

≥90% (HPCE)

assay

-

assay

≥90% (HPCE)

assay

≥90% (HPCE)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

-

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100


Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–2 of 2 Questions  
  1. What is the extinction coefficient for product 53608.

    1 answer
    1. Although the excitation and emission wavelengths are measured during quality control testing, there is no testing to determine the extinction coefficient for 53608 (Biotin (5-fluorescein) conjugate). There was no information on file regarding the extinction coefficient for product 53608.

      Helpful?

  2. what are the solvents required to dilute these two reagents and also the recommended concentration?

    1 answer
    1. The solubility of Biotin (5-fluorescein) conjugate 53608-1MG-F is not a specification for product release, but it should be soluble in DMSO at least up to a concentration of 3 mM.

      Helpful?

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