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72091

NBT-BCIP® solution

BioReagent

Synonym(s):

BCIP®/NBT, NBT-X-phosphate

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Pack SizeSKUAvailabilityPrice
2.5 mL

Available to ship TODAYfromMILWAUKEE

$106.00
10 mL

Available to ship TODAYfromMILWAUKEE

$331.00

About This Item

UNSPSC Code:
12171500
PubChem Substance ID:
329763805
NACRES:
NA.21

$106.00

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product line

BioReagent

Quality Level

100

form

liquid

technique(s)

microbe id | staining: suitable

solubility

0.1 M Tris-HCl, pH 7.4: 0.0175 mL/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23.COc4cc(ccc4-[n+]5nc(nn5-c6ccc(cc6)[N+]([O-])=O)-c7ccccc7)-c8ccc(c(OC)c8)-[n+]9nc(nn9-c%10ccc(cc%10)[N+]([O-])=O)-c%11ccccc%11

InChI

1S/C40H30N10O6.C8H6BrClNO4P.C7H9N.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6;;/h3-26H,1-2H3;1-3,11H,(H2,12,13,14);2-5H,8H2,1H3;2*1H/q+2;;;;/p-2

InChI key

GDPIIVBVMSORRQ-UHFFFAOYSA-L

Physical form

solution of 18.8 mg/ml nitro-blue tetrazolium chloride; 9.4 mg/ml 5-bromo-4-chloro-3-indolylphosphate toluidine salt in 67% DMSO

Other Notes

Chromogenic phosphatase substrate producing a blue-colored precipitate at the site of enzymatic activity[1]

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item
B19111168145100194046
NBT-BCIP® solution BioReagent

Sigma-Aldrich

72091

NBT-BCIP® solution

BCIP®/NBT Liquid Substrate System ready to use solution

Sigma-Aldrich

B1911

BCIP®/NBT Liquid Substrate System

NBT/BCIP Stock Solution

Roche

11681451001

NBT/BCIP Stock Solution

Sodium phosphate dibasic solution BioUltra, 0.5 M in H2O

Sigma-Aldrich

94046

Sodium phosphate dibasic solution

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

-

technique(s)

-

solubility

0.1 M Tris-HCl, pH 7.4: 0.0175 mL/mL, clear

solubility

-

solubility

-

solubility

H2O: 0.5 M at 20 °C, clear, colorless

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

application(s)

diagnostic assay manufacturing

form

liquid

form

liquid

form

solution

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

Quality Level

100

Quality Level

300

Quality Level

-

Quality Level

100

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

152.6 °F - closed cup

flash_point_c

67 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB8209
BCBV6055
BCBS2526V
BCBP6799V
BCBN5576V

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Makrina Totsika et al.
Journal of bacteriology, 191(12), 3901-3908 (2009-04-21)
Disulfide bond (DSB) formation is catalyzed by disulfide bond proteins and is critical for the proper folding and functioning of secreted and membrane-associated bacterial proteins. Uropathogenic Escherichia coli (UPEC) strains possess two paralogous disulfide bond systems: the well-characterized DsbAB system
D Thompson et al.
BioTechniques, 12(5), 656-658 (1992-05-01)
A general method is described for the electrophoretic transfer of proteins from stained gels to membranes and subsequent Western detection of specific proteins on the stained membranes. Proteins are separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and the gels
Andrea L H Arnett et al.
Frontiers in microbiology, 2, 220-220 (2011-11-09)
Recombinant adeno-associated viral (rAAV) vectors promote long-term gene transfer in many animal species. Significant effort has focused on the evaluation of rAAV delivery and the immune response in both murine and canine models of neuromuscular disease. However, canines provided for
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Global Trade Item Number

SKUGTIN
72091-10ML04061832855912
72091-2.5ML04061838203298

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