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83702

Rhodamine 123

BioReagent, suitable for fluorescence, ≥85% (HPLC)

Synonyme(s) :

2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester

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A propos de cet article

Formule linéaire :
C21H17ClN2O3
Numéro CAS:
62669-70-9
Poids moléculaire :
380.82
UNSPSC Code:
12352116
NACRES:
NA.32
PubChem Substance ID:
57652966
EC Number:
263-687-8
Beilstein/REAXYS Number:
6030951
MDL number:
MFCD00012664
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product line

BioReagent

Quality Level

100

assay

≥85% (HPLC)

form

powder

impurities

≤10% water

solubility

ethanol: 20 mg/mL, clear, red

suitability

in accordance for fluorescence, suitable for fluorescence

SMILES string

Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24

InChI

1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H

InChI key

MYFATKRONKHHQL-UHFFFAOYSA-N

General description

Rhodamine 123 is a fluorescent dye.

Application

Rhodamine 123 has been used:
  • as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays
  • to label the cells for estimating the P-gp expression by flow cytometry
  • in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)
  • the detection of mitochondrial membrane potential (ΔΨm)

Biochem/physiol Actions

Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.
Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.

Other Notes

Supravital mitochondrial-specific dye

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Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
BCCN7702
BCCM6618
BCCL3983
BCCH9222
BCCG4466

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The use of the supravital mitochondrial-specific dye Rhodamine 123 (Rh 123) in combination with flow cytometry permits the monitoring of the changes in the mitochondrial transmembrane potential, reflecting the overall mitochondrial activity of the living cell. While this probe appears
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Numéro d'article de commerce international

RéférenceGTIN
83702-50MG04061833005798
83702-10MG04061833005781