I accidentally bought this thinking it was MTT salt to use in a cell proliferation assay but it is MTT Formazan, the water insoluble end product! Therefore, it is useless for the assay. I suppose you could use it as a control to optimize your buffer for solubilizing the insoluble Formazan. The description for this product is misleading and should clarify that this is not what you need for an MTT assay, and refer the customer to MTT salt. That said, this is a nice purple crystalline powder and insoluble in water consistent with MTT Formazan, if that is what you actually want.
88417
MTT Formazan
MTT reduction end product, powder
MTT Formazan
Synonym(s):
1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan
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| Size/SKU | Availability | Price |
|---|---|---|
250 mg | Available to ship TODAYfromMILWAUKEE | $60.10 |
1 g | Available to ship TODAYfromMILWAUKEE | $193.00 |
About This Item
Empirical Formula (Hill Notation):
C18H17N5S
CAS Number:
Molecular Weight:
335.43
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
$60.10
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Product Name
MTT Formazan, MTT reduction end product
Quality Segment
form
powder
solubility
chloroform: 10 mg/mL, clear, dark violet
SMILES string
Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
InChI
1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
InChI key
GIFKVQOPAJDJLO-SZVLODPRSA-N
Biochem/physiol Actions
MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.
Other Notes
Reviews: Tetrazolium salts and formazans
1 of 1
This Item | |||
|---|---|---|---|
| description MTT reduction end product | description powder | description crystalline | description 98% |
| solubility chloroform: 10 mg/mL, clear, dark violet | solubility acetone: 1 mg/mL | solubility DMF: 50 mg/mL | solubility 2-methoxyethanol: soluble 20 mg/mL, H2O: 5 mg/mL |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| form powder | form powder | form crystalline | form powder |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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