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Anti-ATG5 (C-terminal) antibody produced in rabbit

Name: Anti-ATG5 (C-terminal) antibody produced in rabbit
Brand: SIGMA
Price: 552 USD
Availability: InStock

~1 mg/mL, affinity isolated antibody

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Synonym(s):

Anti-Atg5 (C-terminal) antibody produced in rabbit, Anti-ASP, Anti-Apg5, Anti-Apg5L, Anti-Apoptosis-specific protein, Anti-Autophagy protein 5-like

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$552.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD09265351

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ICC, WB

Citations:

$552.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen ~56 kDa

species reactivity

mouse, human

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunocytochemistry: suitable, western blot: 1-2 μg/mL using whole extract of mouse liver or human U87 cells

UniProt accession no.

Q9H1Y0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATG5(9474)
mouse ... Atg5(11793)

Description

General description

ATG5 was found localized to double FYVE domain containing protein 1 (DFCP1)- specific omegasomes.

ATG5 (autophagy related 5) is located on human chromosome band 6q21.

Macroautophagy, usually referred to as autophagy, is a major pathway for bulk degradation of cytoplasmic constituents and organelles. Autophagy is an evolutionary conserved pathway seen in all eukaryotic cells. At least 16 ATG genes, required for autophagosome formation were identified in yeast by genetic screens. For many of these genes, related homologs have been identified in mammals. Atg5, also known as Apg5, is a 32 kDa protein essential for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. This conjugation reaction requires ATP and two enzymes, Atg7 and Atg10, which are E1- and E2-like enzymes, respectively. The Atg12-Atg5 conjugate interacts non-covalently with Atg16. The Atg12-Atg5-Atg16 complex localizes to autophagosome precursors and plays an essential role in autophagosome formation. It has been reported that in addition to the role of Atg5 in the formation of autophagosomes, an Atg5 fragment produced by calpain cleavage has pro-apoptotic properties.

Rabbit polyclonal anti-ATG5 (C-terminal) antibody recognizes mouse and human Atg5-Atg12 complex by immunoblotting, ~56 kDa. Detection of the Atg5-Atg12 complex band by immunoblotting is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 262-275 of mouse Atg5, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence differs by one amino acid in human.

Application

Anti-ATG5 (C-terminal) antibody has been used in immunohistochemical (IHC) staining.

Rabbit anti-ATG5 (C-terminal) antibody has been used for western blot analysis in U2OS cells. The antibody has also been used for immunoblot assays in CT26 and MCA205 cells.

Rabbit polyclonal anti-ATG5 (C-terminal) antibody is used to tag Atg5-Atg12 complex for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Atg5-Atg12 complex in the formation and function of autophagosomes. Rabbit anti-ATG5 was used as primary antibody for immunocytochemistry on HEK293 and Cos7 cells. Cells were fixed in 3.7% formaldehyde in 200 mM Hepes prior to antibody treatment.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	MAB2605	WH0064422M6	SAB4200100
Sigma-Aldrich A0731 Anti-ATG5 (C-terminal) antibody produced in rabbit	Sigma-Aldrich MAB2605 Anti-ATG5 Antibody, clone 177.19	Sigma-Aldrich WH0064422M6 Monoclonal Anti-ATG3 antibody produced in mouse	Sigma-Aldrich SAB4200100 Anti-ATG13 antibody produced in rabbit
description ~1 mg/mL, affinity isolated antibody	description clone 177.19, from mouse	description clone 1F7, purified immunoglobulin, buffered aqueous solution	description ~1.0 mg/mL, affinity isolated antibody
Quality Level 200	Quality Level 100	Quality Level 100	Quality Level -
antibody form affinity isolated antibody	antibody form purified antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source mouse	biological source mouse	biological source rabbit
storage temp. −20°C	storage temp. -	storage temp. −20°C	storage temp. −20°C
UniProt accession no. Q9H1Y0	UniProt accession no. Q9H1Y0	UniProt accession no. Q9NT62	UniProt accession no. -

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